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1000 Titel
  • In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C
1000 Autor/in
  1. Jäkel, Helen |
  2. Scheinpflug, Kathi |
  3. Mühldorfer, Kristin |
  4. Gianluppi, Rafael |
  5. Schardong Lucca, Matheus |
  6. Mellagi, Ana Paula |
  7. Bortolozzo, Fernando |
  8. Waberski, Dagmar |
1000 Erscheinungsjahr 2021
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-01-11
1000 Erschienen in
1000 Quellenangabe
  • 12:9
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/s40104-020-00530-6 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798330/ |
1000 Ergänzendes Material
  • https://jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00530-6#Sec25 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Hypothermic preservation of boar semen is considered a potential method for omitting antibiotics from insemination doses, thereby contributing to the global antibiotic resistance defence strategy. The main challenges are chilling injury to spermatozoa and bacterial growth during semen storage leading to reduced fertility. OBJECTIVES: To examine chilling injury and the number and type of bacteria in boar semen stored at 5 °C in the absence of antibiotics, and to assess the applicability of hypothermic semen storage under field conditions. MATERIAL AND METHODS: Boar ejaculates were extended with AndroStar® Premium, stored at 17 °C with and at 5 °C without antibiotics and tested for functional sperm parameters by flow cytometry. Raw semen and extended samples were investigated bacteriologically. Fertility was evaluated after once-daily inseminations of 194 sows in a field study. RESULTS: Lethal sperm damage assessed by motility and membrane integrity was low throughout storage in both experimental groups. Sublethal chilling effects based on the decrease of viable spermatozoa with low membrane fluidity were higher (P < 0.05) up until 72 h in sperm stored at 5 °C compared to 17 °C but did not differ after 144 h. After 72 h, incubation in capacitating medium for 60 min induced a similar decrease in viable sperm with high mitochondria membrane potential and low cytosolic calcium in both groups. In semen stored at 5 °C, bacteria counts were below 103 CFU/mL and the bacteria spectrum was similar to that of raw semen. In 88% of 34 boars, cooled semen fulfilled the requirements for insemination. Fertility was high and did not differ (P > 0.05) between sow groups inseminated with semen stored antibiotic-free at 5 °C and semen stored at 17 °C with antibiotics. CONCLUSION: Despite subtle chilling effects and low bacterial numbers, antibiotic-free hypothermic storage of boar semen offers the possibility to reduce the use of antibiotics in pig insemination. However, strict sanitary guidelines must be maintained and further evidence of efficiency under field conditions is considered desirable.
1000 Sacherschließung
lokal Boar semen
lokal Bacteria
lokal Semen preservation
lokal Chilling
lokal Antibiotics
lokal Fertility
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0003-0060-3103|https://frl.publisso.de/adhoc/uri/U2NoZWlucGZsdWcsIEthdGhp|https://orcid.org/0000-0001-8023-4736|https://orcid.org/0000-0001-8250-5210|https://orcid.org/0000-0003-3243-8966|https://orcid.org/0000-0002-0296-5637|https://orcid.org/0000-0001-5302-328X|https://orcid.org/0000-0001-6616-2035
1000 Label
1000 Förderer
  1. Förderverein Bioökonomieforschung (FBF) |
  2. Landwirtschaftliche Rentenbank |
  3. Bundesanstalt für Landwirtschaft und Ernährung |
  4. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
  5. Deutscher Akademischer Austauschdienst |
  6. Project DEAL |
1000 Fördernummer
  1. -
  2. -
  3. 28-RZ-3.054, 28-RZ-3.051
  4. 57390778; 88887.185883/2018-00
  5. 57390778; 88887.185883/2018-00
  6. -
1000 Förderprogramm
  1. -
  2. -
  3. AMIKOS
  4. PROBAL
  5. PROBAL
  6. Open Access funding
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Förderverein Bioökonomieforschung (FBF) |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Landwirtschaftliche Rentenbank |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Bundesanstalt für Landwirtschaft und Ernährung |
    1000 Förderprogramm AMIKOS
    1000 Fördernummer 28-RZ-3.054, 28-RZ-3.051
  4. 1000 joinedFunding-child
    1000 Förderer Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
    1000 Förderprogramm PROBAL
    1000 Fördernummer 57390778; 88887.185883/2018-00
  5. 1000 joinedFunding-child
    1000 Förderer Deutscher Akademischer Austauschdienst |
    1000 Förderprogramm PROBAL
    1000 Fördernummer 57390778; 88887.185883/2018-00
  6. 1000 joinedFunding-child
    1000 Förderer Project DEAL |
    1000 Förderprogramm Open Access funding
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6427158.rdf
1000 Erstellt am 2021-04-29T14:04:00.732+0200
1000 Erstellt von 122
1000 beschreibt frl:6427158
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Fri Apr 30 09:06:54 CEST 2021
1000 Objekt bearb. Fri Apr 30 09:05:59 CEST 2021
1000 Vgl. frl:6427158
1000 Oai Id
  1. oai:frl.publisso.de:frl:6427158 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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