Download
a-1440-0278.pdf 1,33MB
WeightNameValue
1000 Titel
  • Adrenal Hormone Interactions and Metabolism: A Single Sample Multi-Omics Approach
1000 Autor/in
  1. Bechmann, Nicole |
  2. Watts, Deepika |
  3. Steenblock, Charlotte |
  4. Wallace, Paal William |
  5. Schürmann, Annette |
  6. Bornstein, Stefan R. |
  7. Wielockx, Ben |
  8. Eisenhofer, Graeme |
  9. Peitzsch, Mirko |
1000 Erscheinungsjahr 2021
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-04-26
1000 Erschienen in
1000 Quellenangabe
  • 53(05):326-334
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1055/a-1440-0278 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105089/ |
1000 Ergänzendes Material
  • https://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-1440-0278#N69307 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The adrenal gland is important for many physiological and pathophysiological processes, but studies are often restricted by limited availability of sample material. Improved methods for sample preparation are needed to facilitate analyses of multiple classes of adrenal metabolites and macromolecules in a single sample. A procedure was developed for preparation of chromaffin cells, mouse adrenals, and human chromaffin tumors that allows for multi-omics analyses of different metabolites and preservation of native proteins. To evaluate the new procedure, aliquots of samples were also prepared using conventional procedures. Metabolites were analyzed by liquid-chromatography with mass spectrometry or electrochemical detection. Metabolite contents of chromaffin cells and tissues analyzed with the new procedure were similar or even higher than with conventional methods. Catecholamine contents were comparable between both procedures. The TCA cycle metabolites, cis-aconitate, isocitate, and α-ketoglutarate were detected at higher concentrations in cells, while in tumor tissue only isocitrate and potentially fumarate were measured at higher contents. In contrast, in a broad untargeted metabolomics approach, a methanol-based preparation procedure of adrenals led to a 1.3-fold higher number of detected metabolites. The established procedure also allows for simultaneous investigation of adrenal hormones and related enzyme activities as well as proteins within a single sample. This novel multi-omics approach not only minimizes the amount of sample required and overcomes problems associated with tissue heterogeneity, but also provides a more complete picture of adrenal function and intra-adrenal interactions than previously possible.
1000 Sacherschließung
lokal adrenal
lokal TCA-cycle metabolites
lokal catecholamines
lokal preservation of native proteins
lokal cortical-medullary interaction
lokal steroids
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-6932-333X|https://frl.publisso.de/adhoc/uri/V2F0dHMsIERlZXBpa2E=|https://orcid.org/0000-0002-9635-4860|https://frl.publisso.de/adhoc/uri/V2FsbGFjZSwgUGFhbCBXaWxsaWFt|https://frl.publisso.de/adhoc/uri/U2Now7xybWFubiwgQW5uZXR0ZQ==|https://frl.publisso.de/adhoc/uri/Qm9ybnN0ZWluLCBTdGVmYW4gUi4=|https://frl.publisso.de/adhoc/uri/V2llbG9ja3gsIEJlbg==|https://frl.publisso.de/adhoc/uri/RWlzZW5ob2ZlciwgR3JhZW1l|https://frl.publisso.de/adhoc/uri/UGVpdHpzY2gsIE1pcmtv
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Bundesministerium für Bildung und Forschung |
  3. Brandenburg State |
1000 Fördernummer
  1. 314061271
  2. -
  3. 82DZD00302
1000 Förderprogramm
  1. CRC/Transregio 205/1; INST 515/28–1 FUGG
  2. -
  3. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm CRC/Transregio 205/1; INST 515/28–1 FUGG
    1000 Fördernummer 314061271
  2. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Brandenburg State |
    1000 Förderprogramm -
    1000 Fördernummer 82DZD00302
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6427665.rdf
1000 Erstellt am 2021-05-25T11:08:45.416+0200
1000 Erstellt von 25
1000 beschreibt frl:6427665
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet 2021-05-25T11:12:07.710+0200
1000 Objekt bearb. Tue May 25 11:09:39 CEST 2021
1000 Vgl. frl:6427665
1000 Oai Id
  1. oai:frl.publisso.de:frl:6427665 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source