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1000 Titel
  • Modulation of the intrinsic chromatin binding property of HIV-1 integrase by LEDGF/p75
1000 Autor/in
  1. Lapaillerie, Delphine |
  2. Lelandais, Benoît |
  3. Mauro, Eric |
  4. Lagadec, Floriane |
  5. Tumiotto, Camille |
  6. Miskey, Csaba |
  7. Ferran, Guillaume |
  8. Kuschner, Natacha |
  9. Calmels, Christina |
  10. Métifiot, Mathieu |
  11. Rooryck, Caroline |
  12. Ivics, Zoltan |
  13. Ruff, Marc |
  14. Zimmer, Christophe |
  15. Lesbats, Paul |
  16. Toutain, Jérôme |
  17. Parissi, Vincent |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-10-11
1000 Erschienen in
1000 Quellenangabe
  • 49(19):11241-11256
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1093/nar/gkab886 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565322/ |
1000 Ergänzendes Material
  • https://academic.oup.com/nar/article/49/19/11241/6389624?login=false#312006116 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The stable insertion of the retroviral genome into the host chromosomes requires the association between integration complexes and cellular chromatin via the interaction between retroviral integrase and the nucleosomal target DNA. This final association may involve the chromatin-binding properties of both the retroviral integrase and its cellular cofactor LEDGF/p75. To investigate this and better understand the LEDGF/p75-mediated chromatin tethering of HIV-1 integrase, we used a combination of biochemical and chromosome-binding assays. Our study revealed that retroviral integrase has an intrinsic ability to bind and recognize specific chromatin regions in metaphase even in the absence of its cofactor. Furthermore, this integrase chromatin-binding property was modulated by the interaction with its cofactor LEDGF/p75, which redirected the enzyme to alternative chromosome regions. We also better determined the chromatin features recognized by each partner alone or within the functional intasome, as well as the chronology of efficient LEDGF/p75-mediated targeting of HIV-1 integrase to chromatin. Our data support a new chromatin-binding function of integrase acting in concert with LEDGF/p75 for the optimal association with the nucleosomal substrate. This work also provides additional information about the behavior of retroviral integration complexes in metaphase chromatin and the mechanism of action of LEDGF/p75 in this specific context.
1000 Sacherschließung
gnd 4203852-2 HIV-Infektion
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/TGFwYWlsbGVyaWUsIERlbHBoaW5l|https://frl.publisso.de/adhoc/uri/TGVsYW5kYWlzLCBCZW5vw650|https://frl.publisso.de/adhoc/uri/TWF1cm8sIEVyaWM=|https://frl.publisso.de/adhoc/uri/TGFnYWRlYywgRmxvcmlhbmU=|https://frl.publisso.de/adhoc/uri/VHVtaW90dG8sIENhbWlsbGU=|https://orcid.org/0000-0002-7566-9556|https://frl.publisso.de/adhoc/uri/IEZlcnJhbiwgR3VpbGxhdW1l|https://frl.publisso.de/adhoc/uri/IEt1c2NobmVyLCBOYXRhY2hh|https://frl.publisso.de/adhoc/uri/Q2FsbWVscywgQ2hyaXN0aW5h|https://frl.publisso.de/adhoc/uri/IE3DqXRpZmlvdCwgTWF0aGlldQ==|https://frl.publisso.de/adhoc/uri/IFJvb3J5Y2ssIENhcm9saW5l|https://orcid.org/0000-0002-7803-6658|https://frl.publisso.de/adhoc/uri/UnVmZiwgTWFyYw==|https://frl.publisso.de/adhoc/uri/IFppbW1lciwgQ2hyaXN0b3BoZQ==|https://frl.publisso.de/adhoc/uri/TGVzYmF0cywgUGF1bA==|https://frl.publisso.de/adhoc/uri/VG91dGFpbiwgSsOpcsO0bWU=|https://orcid.org/0000-0003-1661-7841
1000 Label
1000 Förderer
  1. Agence Nationale de Recherches sur le Sida et les Hépatites Virales |
  2. Centre National de la Recherche Scientifique |
1000 Fördernummer
  1. ECTZ115893; 16-1AEQ-10465
  2. -
1000 Förderprogramm
  1. SIDACTION
  2. Open access funding
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Agence Nationale de Recherches sur le Sida et les Hépatites Virales |
    1000 Förderprogramm SIDACTION
    1000 Fördernummer ECTZ115893; 16-1AEQ-10465
  2. 1000 joinedFunding-child
    1000 Förderer Centre National de la Recherche Scientifique |
    1000 Förderprogramm Open access funding
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6432011.rdf
1000 Erstellt am 2022-03-07T13:27:04.757+0100
1000 Erstellt von 323
1000 beschreibt frl:6432011
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Fri Mar 18 07:35:50 CET 2022
1000 Objekt bearb. Fri Mar 18 07:35:35 CET 2022
1000 Vgl. frl:6432011
1000 Oai Id
  1. oai:frl.publisso.de:frl:6432011 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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