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1000 Titel
  • Development of a Sensitive Detection Method for Alphaviruses and Its Use as a Virus Neutralization Assay
1000 Autor/in
  1. Schmidt, Christin |
  2. Perkovic, Mario |
  3. Schnierle, Barbara |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-06-22
1000 Erschienen in
1000 Quellenangabe
  • 13(7):1191
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3390/v13071191 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310071/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Alphaviruses have a single-stranded, positive-sense RNA genome that contains two open reading frames encoding either the non-structural or the structural genes. Upon infection, the genomic RNA is translated into the non-structural proteins (nsPs). NsPs are required for viral RNA replication and transcription driven from the subgenomic promoter (sgP). Transfection of an RNA encoding the luciferase gene under the control of the sgP into cells enabled the detection of replication-competent chikungunya virus (CHIKV) or Mayaro virus (MAYV) with high sensitivity as a function of the induced luciferase activity. This assay principle was additionally used to analyze virus-neutralizing antibodies in sera and might be an alternative to standard virus neutralization assays based on virus titration or the use of genetically modified tagged viruses.
1000 Sacherschließung
lokal Ross River virus
lokal Mayaro virus
gnd 4188401-2 Virusinfektion
lokal chikungunya virus
lokal subgenomic promoter
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0001-9151-1970|https://orcid.org/0000-0002-8969-5157|https://orcid.org/0000-0001-9563-4564
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1000 Dateien
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1000 Erstellt am 2022-03-15T10:03:06.629+0100
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