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Quadruple mutation GCAC1809-1812TTCT acts as a biomarker in healthy European HBV carriers

  1. Peiffer, Kai-Henrik ORCID logo
  2. Spengler, Catrina
  3. Basic, Michael ORCID logo
  4. Jiang, Bingfu ORCID logo
  5. Kuhnhenn, Lisa
  6. Obermann, Wiebke
  7. Zahn, Tobias
  8. Glitscher, Mirco ORCID logo
  9. Loglio, Alessandro ORCID logo
  10. Facchetti, Floriana ORCID logo
  11. Carra, Gert ORCID logo
  12. Kubesch, Alica ORCID logo
  13. Vermehren, Johannes
  14. Knop, Viola ORCID logo
  15. Graf, Christiana
  16. Dietz, Julia ORCID logo
  17. Finkelmeier, Fabian ORCID logo
  18. Herrmann, Eva ORCID logo
  19. Trebicka, Jonel ORCID logo
  20. Grünweller, Arnold ORCID logo
  21. Zeuzem, Stefan ORCID logo
  22. Sarrazin, Christoph ORCID logo
  23. Lampertico, Pietro ORCID logo
  24. hildt, eberhard ORCID logo

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WeightNameValue
1000 Titel
  • Quadruple mutation GCAC1809-1812TTCT acts as a biomarker in healthy European HBV carriers
1000 Autor/in
  1. Peiffer, Kai-Henrik |
  2. Spengler, Catrina |
  3. Basic, Michael |
  4. Jiang, Bingfu |
  5. Kuhnhenn, Lisa |
  6. Obermann, Wiebke |
  7. Zahn, Tobias |
  8. Glitscher, Mirco |
  9. Loglio, Alessandro |
  10. Facchetti, Floriana |
  11. Carra, Gert |
  12. Kubesch, Alica |
  13. Vermehren, Johannes |
  14. Knop, Viola |
  15. Graf, Christiana |
  16. Dietz, Julia |
  17. Finkelmeier, Fabian |
  18. Herrmann, Eva |
  19. Trebicka, Jonel |
  20. Grünweller, Arnold |
  21. Zeuzem, Stefan |
  22. Sarrazin, Christoph |
  23. Lampertico, Pietro |
  24. hildt, eberhard |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-10-15
1000 Erschienen in
1000 Quellenangabe
  • 5(22):e135833
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1172/jci.insight.135833 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710305 |
1000 Ergänzendes Material
  • https://insight.jci.org/articles/view/135833#sd |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Many mutation analyses of the HBV genome have been performed in the search for new prognostic markers. However, the Kozak sequence preceding precore was covered only infrequently in these analyses. In this study, the HBV core promoter/precore region was sequenced in serum samples from European inactive HBV carriers. Quadruple mutation GCAC1809-1812TTCT was found with a high prevalence of 42% in the Kozak sequence preceding precore among all HBV genotypes. GCAC1809-1812TTCT was strongly associated with coexistence of basal core promoter (BCP) double mutation A1762T/G1764A and lower HBV DNA levels. In vitro GCAC1809-1812TTCT lead to drastically diminished synthesis of pregenomic RNA (pgRNA), precore mRNA, core, HBsAg, and HBeAg. Calculation of the pgRNA secondary structure suggests a destabilization of the pgRNA structure by A1762T/G1764A that was compensated by GCAC1809-1812TTCT. In 125 patients with HBV-related cirrhosis, GCAC1809-1812TTCT was not detected. While a strong association of GCAC1809-1812TTCT with inactive carrier status was observed, BCP double mutation was strongly correlated with cirrhosis, but this was only observed in absence of GCAC1809-1812TTCT. In conclusion, our data reveal that GCAC1809-1812TTCT is highly prevalent in inactive carriers and acts as a compensatory mutation for BCP double mutation. GCAC1809-1812TTCT seems to be a biomarker of good prognosis in HBV infection.
1000 Sacherschließung
gnd 4159548-8 Hepatitis B
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-3757-4476|https://d-nb.info/gnd/1217737294|https://orcid.org/0000-0001-6339-1262|https://orcid.org/0000-0001-8020-0042|https://d-nb.info/gnd/1162343702|https://frl.publisso.de/adhoc/uri/IE9iZXJtYW5uLCBXaWVia2UgICAgICA=|https://d-nb.info/gnd/1216847819|https://orcid.org/0000-0002-8799-7565|https://orcid.org/0000-0001-8022-8803|https://orcid.org/0000-0001-5252-4744|https://orcid.org/0000-0002-7836-2162|https://orcid.org/0000-0002-8627-6088|https://d-nb.info/gnd/1228163960|https://orcid.org/0000-0002-7434-5218|https://d-nb.info/gnd/1129478351|https://orcid.org/0000-0001-6990-3257|https://orcid.org/0000-0001-8559-9910|https://orcid.org/0000-0002-0662-6675|https://orcid.org/0000-0002-7028-3881|https://orcid.org/0000-0003-1508-3016|https://orcid.org/0000-0002-0845-6833|https://orcid.org/0000-0002-2207-9157|https://orcid.org/0000-0002-1026-7476|https://orcid.org/0000-0002-3020-9564
1000 Label
1000 Förderer
  1. Deutsches Zentrum für Infektionsforschung |
  2. Deutsche Forschungsgemeinschaft |
  3. LOEWE Center DRUID |
  4. Ellen Schairer-Stiftung |
  5. Scolari Stiftung |
1000 Fördernummer
  1. -
  2. PE 2403/2-1; HI 25 858/12-1
  3. -
  4. -
  5. -
1000 Förderprogramm
  1. TTU Hepatitis
  2. -
  3. -
  4. -
  5. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsches Zentrum für Infektionsforschung |
    1000 Förderprogramm TTU Hepatitis
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer PE 2403/2-1; HI 25 858/12-1
  3. 1000 joinedFunding-child
    1000 Förderer LOEWE Center DRUID |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Ellen Schairer-Stiftung |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Scolari Stiftung |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6432738.rdf
1000 Erstellt am 2022-03-31T12:08:51.299+0200
1000 Erstellt von 323
1000 beschreibt frl:6432738
1000 Bearbeitet von 317
1000 Zuletzt bearbeitet Mon Apr 25 14:15:07 CEST 2022
1000 Objekt bearb. Mon Apr 25 14:14:44 CEST 2022
1000 Vgl. frl:6432738
1000 Oai Id
  1. oai:frl.publisso.de:frl:6432738 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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