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1000 Titel
  • Bacteriuria and phenotypic antimicrobial susceptibility testing in 45 min by point-of-care Sysmex PA-100 System: first clinical evaluation
1000 Autor/in
  1. Alonso-Tarrés, Carles |
  2. Benjumea Moreno, Carla |
  3. Navarro, Ferran |
  4. Habison, Aline C. |
  5. Gonzàlez-Bertran, Elisenda |
  6. Blanco, Francisco |
  7. Borràs, Jaume |
  8. Garrigó, Montserrat |
  9. Saker, Jarob |
1000 Verlag Springer Berlin Heidelberg
1000 Erscheinungsjahr 2024
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2024-06-03
1000 Erschienen in
1000 Quellenangabe
  • 43(8):1533-1543
1000 Copyrightjahr
  • 2024
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1007/s10096-024-04862-3 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271345/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • <jats:title>Abstract</jats:title><jats:sec> <jats:title>Purpose</jats:title> <jats:p>This study compared the results of the new Sysmex PA-100 AST System, a point-of-care analyser, with routine microbiology for the detection of urinary tract infections (UTI) and performance of antimicrobial susceptibility tests (AST) directly from urine.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>Native urine samples from 278 female patients with suspected uncomplicated UTI were tested in the Sysmex PA-100 and with reference methods of routine microbiology: urine culture for bacteriuria and disc diffusion for AST.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>The analyser delivered bacteriuria results in 15 min and AST results within 45 min. Sensitivity and specificity for detection of microbiologically confirmed bacteriuria were 84.0% (89/106; 95% CI: 75.6–90.4%) and 99.4% (155/156; 95% CI: 96.5–100%), respectively, for bacterial species within the analyser specifications. These are <jats:italic>Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterococcus faecalis</jats:italic> and <jats:italic>Staphylococcus saprophyticus,</jats:italic> which are common species causing uncomplicated UTI. Overall categorical agreement (OCA) for AST results for the five antimicrobials tested in the Sysmex PA-100 (amoxicillin/clavulanic acid, ciprofloxacin, fosfomycin, nitrofurantoin and trimethoprim) ranged from 85.4% (70/82; 95%CI: 75.9–92.2%) for ciprofloxacin to 96.4% (81/84; 95% CI: 89.9–99.3%) for trimethoprim. The Sysmex PA-100 provided an optimal treatment recommendation in 218/278 cases (78.4%), against 162/278 (58.3%) of clinical decisions.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>This first clinical evaluation of the Sysmex PA-100 in a near-patient setting demonstrated that the analyser delivers phenotypic AST results within 45 min, which could enable rapid initiation of the correct targeted treatment with no further adjustment needed. The Sysmex PA-100 has the potential to significantly reduce ineffective or unnecessary antibiotic prescription in patients with UTI symptoms.</jats:p> </jats:sec>
1000 Sacherschließung
lokal Aged, 80 and over [MeSH]
lokal Antimicrobial resistance
lokal Aged [MeSH]
lokal Bacteriuria/diagnosis [MeSH]
lokal Bacteriuria/drug therapy [MeSH]
lokal Antibiotic susceptibility test (AST)
lokal Bacteria/drug effects [MeSH]
lokal Original Article
lokal Microbial Sensitivity Tests/methods [MeSH]
lokal Sensitivity and Specificity [MeSH]
lokal Urinary tract infection (UTI)
lokal Phenotypic antibiotic susceptibility test (AST)
lokal Urinary Tract Infections/microbiology [MeSH]
lokal Bacteria/isolation
lokal Adolescent [MeSH]
lokal Female [MeSH]
lokal Point-of-Care Systems [MeSH]
lokal Adult [MeSH]
lokal Humans [MeSH]
lokal Microbial Sensitivity Tests/instrumentation [MeSH]
lokal Bacteriuria
lokal Point-of-care
lokal Urinary Tract Infections/drug therapy [MeSH]
lokal Middle Aged [MeSH]
lokal Nanofluidics
lokal Urinary Tract Infections/diagnosis [MeSH]
lokal Bacteriuria/microbiology [MeSH]
lokal Anti-Bacterial Agents/pharmacology [MeSH]
lokal Young Adult [MeSH]
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0003-2527-1643|https://frl.publisso.de/adhoc/uri/QmVuanVtZWEgTW9yZW5vLCBDYXJsYQ==|https://orcid.org/0000-0002-4302-2838|https://frl.publisso.de/adhoc/uri/SGFiaXNvbiwgQWxpbmUgQy4=|https://frl.publisso.de/adhoc/uri/R29uesOgbGV6LUJlcnRyYW4sIEVsaXNlbmRh|https://frl.publisso.de/adhoc/uri/QmxhbmNvLCBGcmFuY2lzY28=|https://orcid.org/0000-0003-0300-5686|https://orcid.org/0000-0003-4431-4733|https://frl.publisso.de/adhoc/uri/U2FrZXIsIEphcm9i
1000 Hinweis
  • DeepGreen-ID: 927a8f6599684ec79d41244e03855bd0 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
1000 Label
1000 Förderer
  1. Sysmex Corporation |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Sysmex Corporation |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
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1000 @id frl:6516357.rdf
1000 Erstellt am 2025-07-04T21:12:53.079+0200
1000 Erstellt von 322
1000 beschreibt frl:6516357
1000 Zuletzt bearbeitet 2025-09-09T17:33:57.035+0200
1000 Objekt bearb. Tue Sep 09 17:33:57 CEST 2025
1000 Vgl. frl:6516357
1000 Oai Id
  1. oai:frl.publisso.de:frl:6516357 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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