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Christine T. Schäff, Jeannine Gruse, Josefine Maciej, Manfred Mielenz, Elisa Wirthgen, Andreas Hoeflich, Marion Schmicke, Ralf Pfuhl, Paulina Jawor, Tadeusz Stefaniak, Harald M. Hammon
Effects of Feeding Milk Replacer Ad Libitum or in Restricted Amounts for the First Five Weeks of Life on the Growth, Metabolic Adaptation, and Immune Status of Newborn Calves
www.plosone.org
www.plosone.org
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(concentrate)Tj
4.921 0 Td
(\(Ka)Tj
0 Tc
1.0374 0 Td
()Tj
-0.004 Tc
0.4139 0 Td
(lber)Tj
1.7631 0 Td
(Start)Tj
2.0976 0 Td
(18/3;)Tj
-35.3137 -1.2983 Td
(Vollkraft)Tj
3.7984 0 Td
(Mischfutterwerke)Tj
7.302 0 Td
(GmbH,)Tj
3.2541 0 Td
(Karsta)Tj
0 Tc
2.16 0 Td
()Tj
-0.004 Tc
0.4138 0 Td
(dt,)Tj
1.27 0 Td
(Germany;)Tj
4.2122 0 Td
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5.2441 0 Td
(shown)Tj
2.8459 0 Td
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0.83 0.64 0.02 0 k
1.0375 0 Td
(Table)Tj
2.4435 0 Td
(1)Tj
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(;)Tj
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(deliv-)Tj
-34.8999 -1.2983 Td
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1.9672 0 Td
(by)Tj
1.1792 0 Td
(an)Tj
1.2019 0 Td
(automated)Tj
4.49 0 Td
(feeder;)Tj
2.9027 0 Td
(Fo)Tj
0 Tc
0.5896 0 Td
()Tj
-0.004 Tc
0.4479 0 Td
(rster-Technik)Tj
5.6919 0 Td
(GmbH,)Tj
3.2541 0 Td
(Engen,)Tj
3.0047 0 Td
(Germany\).)Tj
4.5525 0 Td
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4.3596 0 Td
(intake)Tj
2.6872 0 Td
(of)Tj
-36.3285 -1.3039 Td
(MR)Tj
1.7347 0 Td
(and)Tj
1.7292 0 Td
(concentrates)Tj
5.278 0 Td
(and)Tj
1.7348 0 Td
(daily)Tj
2.1487 0 Td
(amounts)Tj
3.7133 0 Td
(of)Tj
1.0148 0 Td
(MR)Tj
1.7348 0 Td
(and)Tj
1.7292 0 Td
(concentrate)Tj
4.9209 0 Td
(intake)Tj
2.6872 0 Td
(were)Tj
2.126 0 Td
(provided)Tj
3.8154 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
-35.5462 -1.2982 Td
(computer)Tj
4.1045 0 Td
(program)Tj
3.7077 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(feeding)Tj
3.1635 0 Td
(station.)Tj
3.1634 0 Td
(MR)Tj
1.7348 0 Td
(was)Tj
1.6951 0 Td
(prepared)Tj
3.8097 0 Td
(in)Tj
1.0319 0 Td
(portions)Tj
3.5999 0 Td
(of)Tj
1.0148 0 Td
(0.5)Tj
1.3947 0 Td
(liters)Tj
2.1883 0 Td
(and)Tj
1.7291 0 Td
(calves)Tj
-34.8205 -1.2983 Td
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2.1203 0 Td
(allowed)Tj
3.2881 0 Td
(to)Tj
1.0262 0 Td
(drink)Tj
2.4321 0 Td
(2)Tj
0.6973 0 Td
(liters)Tj
2.1883 0 Td
(per)Tj
1.5307 0 Td
(visit.)Tj
2.092 0 Td
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3.3959 0 Td
(MR)Tj
1.7291 0 Td
(was)Tj
1.7008 0 Td
(discarded)Tj
4.0818 0 Td
(to)Tj
1.0262 0 Td
(avoid)Tj
2.4094 0 Td
(uncontrolled)Tj
5.3971 0 Td
(MR)Tj
-35.1153 -1.3039 Td
(intake)Tj
2.6872 0 Td
(of)Tj
1.0148 0 Td
(calves)Tj
2.5625 0 Td
(with)Tj
1.9956 0 Td
(no)Tj
1.2755 0 Td
(right.)Tj
2.3755 0 Td
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5.5218 0 Td
(were)Tj
2.1203 0 Td
(offered)Tj
3.0501 0 Td
(in)Tj
1.0374 0 Td
(portions)Tj
3.5944 0 Td
(of)Tj
1.0148 0 Td
(60)Tj
1.1735 0 Td
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0.686 0 Td
(and)Tj
1.7291 0 Td
(calves)Tj
2.5625 0 Td
(could)Tj
-34.401 -1.2983 Td
(take)Tj
1.8708 0 Td
(several)Tj
2.9367 0 Td
(portions)Tj
3.5943 0 Td
(per)Tj
1.5307 0 Td
(visit,)Tj
2.0976 0 Td
(but)Tj
1.5534 0 Td
(it)Tj
0.7823 0 Td
(was)Tj
1.7008 0 Td
(not)Tj
1.5704 0 Td
(possible)Tj
3.4129 0 Td
(to)Tj
1.0261 0 Td
(discard)Tj
3.1295 0 Td
(refusals.)Tj
3.4582 0 Td
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2.3187 0 Td
(dry)Tj
1.5704 0 Td
(matter)Tj
-32.5528 -1.2983 Td
(intake)Tj
2.6872 0 Td
(and)Tj
1.7291 0 Td
(intake)Tj
2.6872 0 Td
(of)Tj
1.0148 0 Td
(metabolizable)Tj
5.7486 0 Td
(energy)Tj
2.9084 0 Td
(\(ME\))Tj
2.3584 0 Td
(were)Tj
2.126 0 Td
(calculated)Tj
4.2009 0 Td
(according)Tj
4.1896 0 Td
(to)Tj
1.0261 0 Td
(Drackley)Tj
3.8041 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
-34.4804 -1.3039 Td
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1.3379 0 Td
(prevent)Tj
3.2541 0 Td
(anemia,)Tj
3.3733 0 Td
(all)Tj
1.1451 0 Td
(calves)Tj
2.5625 0 Td
(received)Tj
3.5377 0 Td
(1)Tj
0.6973 0 Td
(g)Tj
0.686 0 Td
(of)Tj
1.0148 0 Td
(iron)Tj
1.9105 0 Td
(dextran)Tj
3.2882 0 Td
(\(Ursoferran)Tj
4.9719 0 Td
(150)Tj
1.6498 0 Td
(mg/mL;)Tj
3.4185 0 Td
(7)Tj
0.6917 0 Td
(mL)Tj
1.5817 0 Td
(per)Tj
-35.121 -1.2982 Td
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1.3095 0 Td
(Serumwerk)Tj
4.8132 0 Td
(Bernburg)Tj
4.0195 0 Td
(AG,)Tj
1.8426 0 Td
(Bernburg,)Tj
4.2406 0 Td
(Germany\))Tj
4.3256 0 Td
(during)Tj
2.931 0 Td
(the)Tj
1.4683 0 Td
(first)Tj
1.8029 0 Td
(colostrum)Tj
4.2859 0 Td
(feeding.)Tj
3.3846 0 Td
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-34.4237 -1.2983 Td
(evening)Tj
3.3448 0 Td
(feeding,)Tj
3.3846 0 Td
(the)Tj
1.4626 0 Td
(calves)Tj
2.5569 0 Td
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2.1203 0 Td
(fed)Tj
1.4513 0 Td
(halofuginone)Tj
5.5048 0 Td
(\(Halocur;)Tj
4.0592 0 Td
(Intervet)Tj
3.3732 0 Td
(Productions)Tj
5.1137 0 Td
(S.A.,)Tj
2.0466 0 Td
(Igoville,)Tj
-34.418 -1.3039 Td
(France\))Tj
3.2881 0 Td
(for)Tj
1.3833 0 Td
(7)Tj
0.6973 0 Td
(consecutive)Tj
4.8813 0 Td
(days)Tj
1.9955 0 Td
(\(2)Tj
1.0375 0 Td
(mL/10)Tj
2.8573 0 Td
(kg\))Tj
1.5194 0 Td
(to)Tj
1.0261 0 Td
(prevent)Tj
3.2598 0 Td
(diarrhea)Tj
3.5773 0 Td
(resulting)Tj
3.7247 0 Td
(from)Tj
/F13 1 Tf
2.2054 0 Td
[( )13(\n)-20()-13()12()11(\r)7()10()4(\r)11(\n)-9()4()]TJ
-31.453 -1.2983 Td
[()12()12()]TJ
1.7914 0 Td
[()10()11()]TJ
/F8 1 Tf
(.)Tj
1.7292 0 Td
(Bouts)Tj
2.4888 0 Td
(of)Tj
1.0148 0 Td
(diarrhea)Tj
3.5773 0 Td
(\(scours\))Tj
3.4525 0 Td
(were)Tj
2.1203 0 Td
(treated)Tj
2.9991 0 Td
(using)Tj
2.3924 0 Td
(oral)Tj
1.7745 0 Td
(rehydration)Tj
4.9549 0 Td
(solution)Tj
3.4809 0 Td
(\(Glycostar)Tj
-31.7761 -1.2982 Td
(classic;)Tj
2.9593 0 Td
(WDT)Tj
2.5342 0 Td
(eG,)Tj
1.5817 0 Td
(Garbsen,)Tj
3.8097 0 Td
(Germany\).)Tj
4.5524 0 Td
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1.7802 0 Td
(treatment)Tj
4.1385 0 Td
(days)Tj
1.9956 0 Td
(per)Tj
1.5307 0 Td
(calf)Tj
1.6158 0 Td
(were)Tj
2.1203 0 Td
(recorded)Tj
3.7927 0 Td
(and)Tj
1.7348 0 Td
(are)Tj
-34.1459 -1.2983 Td
(shown)Tj
2.8459 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(results)Tj
2.812 0 Td
(section)Tj
3.0444 0 Td
(and)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(S1)Tj
1.1678 0 Td
(Table)Tj
0 g
(.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 457.6818 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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3.6945 0 Td
(Body)Tj
2.4756 0 Td
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5.7071 0 Td
(and)Tj
1.8662 0 Td
(Tissue)Tj
3.1323 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 440.674 cm
BT
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1 TL
-0.004 Tc
0 0 Td
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1.7801 0 Td
(calves)Tj
2.5625 0 Td
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2.126 0 Td
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3.5376 0 Td
(immediately)Tj
5.1987 0 Td
(after)Tj
2.0353 0 Td
(birth)Tj
2.194 0 Td
(and)Tj
1.7291 0 Td
(then)Tj
2.0182 0 Td
(weekly)Tj
2.948 0 Td
(thereafter.)Tj
4.3087 0 Td
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2.1203 0 Td
(feed)Tj
1.8822 0 Td
(effi-)Tj
-34.4407 -1.2982 Td
(ciency,)Tj
2.9876 0 Td
(namely)Tj
3.1465 0 Td
(body)Tj
2.2167 0 Td
(weight)Tj
2.8856 0 Td
(gain/dry)Tj
3.617 0 Td
(matter)Tj
2.863 0 Td
(intake,)Tj
2.914 0 Td
(body)Tj
2.2167 0 Td
(weight)Tj
2.8856 0 Td
(gain/ME)Tj
3.7247 0 Td
(intake,)Tj
2.914 0 Td
(and)Tj
1.7348 0 Td
(body)Tj
-34.1062 -1.2983 Td
(weight)Tj
2.8856 0 Td
(gain/protein)Tj
5.1987 0 Td
(intake)Tj
2.6872 0 Td
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2.1203 0 Td
(calculated)Tj
4.2066 0 Td
(as)Tj
1.0148 0 Td
(sum)Tj
1.9276 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(entire)Tj
2.5512 0 Td
(experimental)Tj
5.4595 0 Td
(period,)Tj
3.0614 0 Td
(respec-)Tj
-33.596 -1.2982 Td
(tively.)Tj
2.5908 0 Td
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1.7801 0 Td
(calves)Tj
2.5625 0 Td
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2.1204 0 Td
(harvested)Tj
4.0421 0 Td
(prior)Tj
2.2564 0 Td
(to)Tj
1.0261 0 Td
(weaning)Tj
3.5887 0 Td
(on)Tj
1.2756 0 Td
(day)Tj
1.6384 0 Td
(60)Tj
/F14 1 Tf
1.1735 0 Td
[()]TJ
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0.788 0 Td
(2)Tj
0.6917 0 Td
(through)Tj
3.4469 0 Td
(exsanguination)Tj
6.3155 0 Td
(after)Tj
-35.2967 -1.304 Td
(capital)Tj
2.8402 0 Td
(bolt)Tj
1.7745 0 Td
(stunning)Tj
3.7871 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(Research)Tj
3.8041 0 Td
(Institute's)Tj
4.1612 0 Td
(abattoir.)Tj
3.5603 0 Td
(Both)Tj
2.1373 0 Td
(carcass)Tj
3.0274 0 Td
(weight)Tj
2.8856 0 Td
(and)Tj
1.7292 0 Td
(carcass)Tj
3.0273 0 Td
(com-)Tj
-35.24 -1.2982 Td
(position)Tj
3.4922 0 Td
(were)Tj
2.1203 0 Td
(determined)Tj
4.8529 0 Td
(after)Tj
2.0353 0 Td
(cutting)Tj
3.05 0 Td
(and)Tj
1.7291 0 Td
(boning.)Tj
3.2882 0 Td
(Cuts)Tj
2.0693 0 Td
(referred)Tj
3.4242 0 Td
(to)Tj
1.0205 0 Td
(the)Tj
1.474 0 Td
(German)Tj
3.5319 0 Td
(cutting)Tj
-32.0879 -1.2983 Td
(schema)Tj
3.2087 0 Td
(and)Tj
1.7292 0 Td
(are)Tj
1.4513 0 Td
(illustrated)Tj
4.2179 0 Td
(in)Tj
0.83 0.64 0.02 0 k
1.0375 0 Td
(S1)Tj
1.1735 0 Td
(Fig)Tj
0 g
1.4797 0 Td
([)Tj
0.83 0.64 0.02 0 k
(39)Tj
0 g
(].)Tj
2.075 0 Td
(The)Tj
1.7801 0 Td
(weight)Tj
2.8857 0 Td
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1.0148 0 Td
(the)Tj
1.474 0 Td
(cuts,)Tj
2.0522 0 Td
(liver,)Tj
2.211 0 Td
(pancreas,)Tj
3.9572 0 Td
(and)Tj
1.7291 0 Td
(perirenal)Tj
-33.4769 -1.3039 Td
(fat,)Tj
1.4626 0 Td
(was)Tj
1.7008 0 Td
(determined.)Tj
5.074 0 Td
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3.4979 0 Td
(of)Tj
1.0148 0 Td
(liver)Tj
1.9842 0 Td
(tissue)Tj
2.4605 0 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(to)Tj
1.0261 0 Td
(determine)Tj
4.32 0 Td
(the)Tj
1.4684 0 Td
(glycogen)Tj
3.7587 0 Td
(and)Tj
1.7291 0 Td
(glu-)Tj
-35.3591 -1.2983 Td
(cose)Tj
1.9332 0 Td
(concentrations)Tj
6.1795 0 Td
(and)Tj
1.7291 0 Td
(for)Tj
1.3889 0 Td
(mRNA)Tj
3.0785 0 Td
(expression)Tj
4.4673 0 Td
(studies.)Tj
3.2145 0 Td
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1.7801 0 Td
(tissue)Tj
2.4605 0 Td
(was)Tj
1.6951 0 Td
(stored)Tj
2.7099 0 Td
(at)Tj
0.9524 0 Td
(-80C)Tj
2.5796 0 Td
(until)Tj
-34.1686 -1.2982 Td
(further)Tj
3.0273 0 Td
(analysis.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 294.9732 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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5.2724 0 Td
(and)Tj
1.8662 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 278.022 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
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2.5851 0 Td
(samples)Tj
3.3846 0 Td
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3.4413 0 Td
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3.05 0 Td
(vein)Tj
1.9162 0 Td
(puncture)Tj
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(first)Tj
1.8029 0 Td
(feeding,)Tj
3.3902 0 Td
(at)Tj
0.9524 0 Td
(24)Tj
1.1679 0 Td
(h)Tj
0.7483 0 Td
(after)Tj
-34.8942 -1.3039 Td
(the)Tj
1.4683 0 Td
(first)Tj
1.8085 0 Td
(feeding,)Tj
3.3845 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(8th)Tj
1.5251 0 Td
(day)Tj
1.6384 0 Td
(of)Tj
1.0148 0 Td
(life)Tj
1.44 0 Td
(and)Tj
1.7348 0 Td
(weekly)Tj
2.948 0 Td
(thereafter)Tj
4.0762 0 Td
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1.3889 0 Td
(up)Tj
1.2643 0 Td
(to)Tj
1.0261 0 Td
(8)Tj
0.6973 0 Td
(weeks.)Tj
2.829 0 Td
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2.5909 0 Td
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-33.579 -1.2982 Td
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(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(morning)Tj
3.7474 0 Td
(\(7.00)Tj
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(am\))Tj
1.8142 0 Td
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2.7439 0 Td
(start)Tj
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3.1635 0 Td
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1.7858 0 Td
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3.7417 0 Td
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1.0318 0 Td
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2.3414 0 Td
(\(Vacuette;)Tj
4.3086 0 Td
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3.3392 0 Td
(Bio-One)Tj
3.6453 0 Td
(GmbH,)Tj
3.2542 0 Td
(Kremsmu)Tj
0 Tc
3.5433 0 Td
()Tj
-0.004 Tc
0.4535 0 Td
(nster,)Tj
2.4491 0 Td
(Austria\))Tj
3.5036 0 Td
(contain-)Tj
-33.732 -1.3039 Td
(ing:)Tj
1.7234 0 Td
(1.22)Tj
2.3924 0 Td
(mg/mL)Tj
3.1918 0 Td
(K)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 279.8362 224.3338 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(3)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 285.5622 225.9779 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(ethylenediaminetetraac)Tj
9.3202 0 Td
(etic)Tj
1.6328 0 Td
(acid)Tj
1.8708 0 Td
(\(EDTA\))Tj
3.4979 0 Td
(to)Tj
1.0262 0 Td
(determine)Tj
4.3256 0 Td
(the)Tj
1.4684 0 Td
(plasma)Tj
3.033 0 Td
(con-)Tj
-34.7298 -1.2982 Td
(centrations)Tj
4.6999 0 Td
(of)Tj
1.0148 0 Td
(insulin,)Tj
3.2143 0 Td
(IGF-I,)Tj
2.7156 0 Td
(and)Tj
1.7291 0 Td
(IGFBPs;)Tj
3.532 0 Td
(24)Tj
1.6894 0 Td
(mg/mL)Tj
3.1918 0 Td
(sodium)Tj
3.2258 0 Td
(fluoride)Tj
3.3789 0 Td
(and)Tj
1.7348 0 Td
(1.22)Tj
2.3867 0 Td
(mg/mL)Tj
3.1918 0 Td
(K)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 563.811 211.3511 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
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