Transcriptomic profiles of aging in purified human immune cells

  1. Reynolds, Lindsay M
  2. Ding, Jingzhong
  3. Taylor, Jackson R
  4. Lohman, Kurt
  5. Soranzo, Nicola
  6. de la Fuente, Alberto
  7. Liu, Tie Fu
  8. Johnson, Craig
  9. Barr, R Graham
  10. Register, Thomas C
  11. Donohue, Kathleen M
  12. Barr, R Graham
  13. Register, Thomas C
  14. Donohue, Kathleen M
  15. Talor, Monica V
  16. Cihakova, Daniela
  17. Gu, Charles
  18. Divers, Jasmin
  19. Siscovick, David
  20. Burke, Gregory
  21. Post, Wendy
  22. Shea, Steven
  23. Jacobs, David R Jr
  24. Hoeschele, Ina
  25. McCall, Charles E
  26. Kritchevsky, Stephen B
  27. Herrington, David
  28. Tracy, Russell P
  29. Liu, Yongmei

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WeightNameValue
1000 Titel
  • Transcriptomic profiles of aging in purified human immune cells
1000 Autor/in
  1. Reynolds, Lindsay M |
  2. Ding, Jingzhong |
  3. Taylor, Jackson R |
  4. Lohman, Kurt |
  5. Soranzo, Nicola |
  6. de la Fuente, Alberto |
  7. Liu, Tie Fu |
  8. Johnson, Craig |
  9. Barr, R Graham |
  10. Register, Thomas C |
  11. Donohue, Kathleen M |
  12. Barr, R Graham |
  13. Register, Thomas C |
  14. Donohue, Kathleen M |
  15. Talor, Monica V |
  16. Cihakova, Daniela |
  17. Gu, Charles |
  18. Divers, Jasmin |
  19. Siscovick, David |
  20. Burke, Gregory |
  21. Post, Wendy |
  22. Shea, Steven |
  23. Jacobs, David R Jr |
  24. Hoeschele, Ina |
  25. McCall, Charles E |
  26. Kritchevsky, Stephen B |
  27. Herrington, David |
  28. Tracy, Russell P |
  29. Liu, Yongmei |
1000 Erscheinungsjahr 2015
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-04-22
1000 Erschienen in
1000 Quellenangabe
  • 16:333
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2015
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1186/s12864-015-1522-4 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417516/ |
1000 Ergänzendes Material
  • https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-015-1522-4#Declarations |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND Transcriptomic studies hold great potential towards understanding the human aging process. Previous transcriptomic studies have identified many genes with age-associated expression levels; however, small samples sizes and mixed cell types often make these results difficult to interpret. - RESULTS Using transcriptomic profiles in CD14+ monocytes from 1,264 participants of the Multi-Ethnic Study of Atherosclerosis (aged 55–94 years), we identified 2,704 genes differentially expressed with chronological age (false discovery rate, FDR ≤ 0.001). We further identified six networks of co-expressed genes that included prominent genes from three pathways: protein synthesis (particularly mitochondrial ribosomal genes), oxidative phosphorylation, and autophagy, with expression patterns suggesting these pathways decline with age. Expression of several chromatin remodeler and transcriptional modifier genes strongly correlated with expression of oxidative phosphorylation and ribosomal protein synthesis genes. 17% of genes with age-associated expression harbored CpG sites whose degree of methylation significantly mediated the relationship between age and gene expression (p < 0.05). Lastly, 15 genes with age-associated expression were also associated (FDR ≤ 0.01) with pulse pressure independent of chronological age. Comparing transcriptomic profiles of CD14+ monocytes to CD4+ T cells from a subset (n = 423) of the population, we identified 30 age-associated (FDR < 0.01) genes in common, while larger sets of differentially expressed genes were unique to either T cells (188 genes) or monocytes (383 genes). At the pathway level, a decline in ribosomal protein synthesis machinery gene expression with age was detectable in both cell types. - CONCLUSIONS An overall decline in expression of ribosomal protein synthesis genes with age was detected in CD14+ monocytes and CD4+ T cells, demonstrating that some patterns of aging are likely shared between different cell types. Our findings also support cell-specific effects of age on gene expression, illustrating the importance of using purified cell samples for future transcriptomic studies. Longitudinal work is required to establish the relationship between identified age-associated genes/pathways and aging-related diseases.
1000 Sacherschließung
lokal Monocyte
lokal Ribonucleoprotein complex
lokal Aging
lokal Protein Synthesis
lokal Transcriptome
lokal Autophagy
lokal Mitochondrial ribosome
lokal Translation
lokal Methylation
lokal T cell
lokal Oxidative phosphorylation
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
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1000 Label
1000 Förderer
  1. National Heart, Lung and Blood Institute |
  2. National Center for Research Resources |
  3. National Institute of Aging |
1000 Fördernummer
  1. N01-HC; R01HL101250
  2. UL1-RR-024156; UL1-RR-025005
  3. T32AG033534
1000 Förderprogramm
  1. -
  2. -
  3. MESA Epigenomics & Transcriptomics Study
1000 Dateien
  1. Transcriptomic profiles of aging in purified human immune cells
    Transcriptomic profiles of aging in purified human immune cells
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer National Heart, Lung and Blood Institute |
    1000 Förderprogramm -
    1000 Fördernummer N01-HC; R01HL101250
  2. 1000 joinedFunding-child
    1000 Förderer National Center for Research Resources |
    1000 Förderprogramm -
    1000 Fördernummer UL1-RR-024156; UL1-RR-025005
  3. 1000 joinedFunding-child
    1000 Förderer National Institute of Aging |
    1000 Förderprogramm MESA Epigenomics & Transcriptomics Study
    1000 Fördernummer T32AG033534
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405273.rdf
1000 Erstellt am 2017-11-06T12:28:45.838+0100
1000 Erstellt von 122
1000 beschreibt frl:6405273
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet 2021-03-30T11:20:07.069+0200
1000 Objekt bearb. Tue Mar 30 11:20:06 CEST 2021
1000 Vgl. frl:6405273
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405273 |
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1000 Sichtbarkeit Daten public
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