1000 Titel
  • Comparative Kinetics of Escherichia coli- and Staphylococcus aureus-Specific Activation of Key Immune Pathways in Mammary Epithelial Cells Demonstrates That S. aureus Elicits a Delayed Response Dominated by Interleukin-6 (IL-6) but Not by IL-1A or Tumor Necrosis Factor Alpha
1000 Autor/in
  1. Günther, Juliane |
  2. Esch, Kathrin |
  3. Poschadel, Norbert |
  4. Petzl, Wolfram |
  5. Zerbe, Holm |
  6. Mitterhuemer, Simone |
  7. Blum, Helmut |
  8. Seyfert, Hans-Martin |
1000 Erscheinungsjahr 2010
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2010-11-29
1000 Erschienen in
1000 Quellenangabe
  • 79(2): 695-707
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3028868/ |
  • https://doi.org/10.1128/IAI.01071-10 |
1000 Ergänzendes Material
  • http://iai.asm.org/content/79/2/695/suppl/DC1 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Infections of the udder by Escherichia coli very often elicit acute inflammation, while Staphylococcus aureus infections tend to cause mild, subclinical inflammation and persistent infections. The molecular causes underlying the different disease patterns are poorly understood. We therefore profiled the kinetics and extents of global changes in the transcriptome of primary bovine mammary epithelial cells (MEC) after challenging them with heat-inactivated preparations of E. coli or S. aureus pathogens. E. coli swiftly and strongly induced an expression of cytokines and bactericidal factors. S. aureus elicited a retarded response and failed to quickly induce an expression of bactericidal factors. Both pathogens induced similar patterns of chemokines for cell recruitment into the udder, but E. coli stimulated their synthesis much faster and stronger. The genes that are exclusively and most strongly upregulated by E. coli may be clustered into a regulatory network with tumor necrosis factor alpha (TNF-α) and interleukin-1 (IL-1) in a central position. In contrast, the expression of these master cytokines is barely regulated by S. aureus. Both pathogens quickly trigger an enhanced expression of IL-6. This is still possible after completely abrogating MyD88-dependent Toll-like receptor (TLR) signaling in MEC. The E. coli-specific strong induction of TNF-α and IL-1 expression may be causative for the severe inflammatory symptoms of animals suffering from E. coli mastitis, while the avoidance to quickly induce the synthesis of bactericidal factors may support the persistent survival of S. aureus within the udder. We suggest that S. aureus subverts the MyD88-dependent activation of immune gene expression in MEC.
1000 Fachgruppe
  1. Medizin |
  2. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R8O8bnRoZXIsIEp1bGlhbmU=|https://frl.publisso.de/adhoc/creator/RXNjaCwgS2F0aHJpbg==|https://frl.publisso.de/adhoc/creator/UG9zY2hhZGVsLCBOb3JiZXJ0|https://frl.publisso.de/adhoc/creator/UGV0emwsIFdvbGZyYW0=|https://frl.publisso.de/adhoc/creator/WmVyYmUsIEhvbG0=|https://frl.publisso.de/adhoc/creator/TWl0dGVyaHVlbWVyLCBTaW1vbmU=|https://frl.publisso.de/adhoc/creator/Qmx1bSwgSGVsbXV0|https://frl.publisso.de/adhoc/creator/U2V5ZmVydCwgSGFucy1NYXJ0aW4=
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
1000 Fördernummer
  1. Se 326/14-3
1000 Förderprogramm
  1. program FOR585
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm program FOR585
    1000 Fördernummer Se 326/14-3
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406573.rdf
1000 Erstellt am 2018-01-30T18:18:33.731+0100
1000 Erstellt von 218
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1000 Zuletzt bearbeitet Thu Jan 30 23:51:20 CET 2020
1000 Objekt bearb. Mon May 07 12:05:46 CEST 2018
1000 Vgl. frl:6406573
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406573 |
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