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Engineering of complex protein sialylation in plants

  1. Kallolimath, Somanath
  2. Castilho, Alexandra
  3. Strasser, Richard
  4. Grünwald-Gruber, Clemens
  5. Altmann, Friedrich
  6. Strubl, Sebastian
  7. Galuska, Christina Elisabeth
  8. Zlatina, Kristina ORCID logo
  9. Galuska, Sebastian Peter
  10. Werner, Stefan
  11. Thiesler, Hauke
  12. Werneburg, Sebastian
  13. Hildebrandt, Herbert
  14. Gerardy-Schahn, Rita
  15. Steinkellner, Herta

WeightNameValue
1000 Titel
  • Engineering of complex protein sialylation in plants
1000 Autor/in
  1. Kallolimath, Somanath |
  2. Castilho, Alexandra |
  3. Strasser, Richard |
  4. Grünwald-Gruber, Clemens |
  5. Altmann, Friedrich |
  6. Strubl, Sebastian |
  7. Galuska, Christina Elisabeth |
  8. Zlatina, Kristina |
  9. Galuska, Sebastian Peter |
  10. Werner, Stefan |
  11. Thiesler, Hauke |
  12. Werneburg, Sebastian |
  13. Hildebrandt, Herbert |
  14. Gerardy-Schahn, Rita |
  15. Steinkellner, Herta |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-07-21
1000 Erschienen in
1000 Quellenangabe
  • 113(34): 9498-9503
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003249/ |
  • https://dx.doi.org/10.1073/pnas.1604371113 |
1000 Ergänzendes Material
  • http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1604371113/-/DCSupplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Sialic acids (Sias) are abundant terminal modifications of protein-linked glycans. A unique feature of Sia, compared with other monosaccharides, is the formation of linear homo-polymers, with its most complex form polysialic acid (polySia). Sia and polySia mediate diverse biological functions and have great potential for therapeutic use. However, technological hurdles in producing defined protein sialylation due to the enormous structural diversity render their precise investigation a challenge. Here, we describe a plant-based expression platform that enables the controlled in vivo synthesis of sialylated structures with different interlinkages and degree of polymerization (DP). The approach relies on a combination of stably transformed plants with transient expression modules. By the introduction of multigene vectors carrying the human sialylation pathway into glycosylation-destructed mutants, transgenic plants that sialylate glycoproteins in α2,6- or α2,3-linkage were generated. Moreover, by the transient coexpression of human α2,8-polysialyltransferases, polySia structures with a DP >40 were synthesized in these plants. Importantly, plant-derived polySia are functionally active, as demonstrated by a cell-based cytotoxicity assay and inhibition of microglia activation. This pathway engineering approach enables experimental investigations of defined sialylation and facilitates a rational design of glycan structures with optimized biotechnological functions.
1000 Sacherschließung
lokal protein polysialylation
lokal Nicotiana benthamiana
lokal glycoengineering
lokal recombinant proteins
lokal in planta sialylation
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S2FsbG9saW1hdGgsIFNvbWFuYXRo|https://frl.publisso.de/adhoc/creator/Q2FzdGlsaG8sIEFsZXhhbmRyYQ==|https://frl.publisso.de/adhoc/creator/U3RyYXNzZXIsIFJpY2hhcmQ=|https://frl.publisso.de/adhoc/creator/R3LDvG53YWxkLUdydWJlciwgQ2xlbWVucw==|https://frl.publisso.de/adhoc/creator/QWx0bWFubiwgRnJpZWRyaWNo|https://frl.publisso.de/adhoc/creator/U3RydWJsLCBTZWJhc3RpYW4=|https://frl.publisso.de/adhoc/creator/R2FsdXNrYSwgQ2hyaXN0aW5hIEVsaXNhYmV0aA==|http://orcid.org/0000-0003-2912-3445|https://frl.publisso.de/adhoc/creator/R2FsdXNrYSwgU2ViYXN0aWFuIFBldGVy|https://frl.publisso.de/adhoc/creator/V2VybmVyLCBTdGVmYW4=|https://frl.publisso.de/adhoc/creator/VGhpZXNsZXIsIEhhdWtl|https://frl.publisso.de/adhoc/creator/V2VybmVidXJnLCBTZWJhc3RpYW4=|https://frl.publisso.de/adhoc/creator/SGlsZGVicmFuZHQsIEhlcmJlcnQ=|https://frl.publisso.de/adhoc/creator/R2VyYXJkeS1TY2hhaG4sIFJpdGE=|https://frl.publisso.de/adhoc/creator/U3RlaW5rZWxsbmVyLCBIZXJ0YQ==
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG)
  2. Behring-Roentgen-Stiftung
  3. Austrian Research Promotion Agency
  4. Icon Genetics GmbH
  5. Austrian Science Fund
  6. Austrian Federal Ministry of Transport, Innovation and Technology
1000 Fördernummer
  1. GA 1755/1-2
  2. -
  3. -
  4. 822757
  5. L575-B13; TRB242 B20; W1224-B09
  6. -
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
  5. -
  6. -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6410553.rdf
1000 Erstellt am 2018-10-12T11:39:13.282+0200
1000 Erstellt von 122
1000 beschreibt frl:6410553
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Thu Jan 30 21:54:58 CET 2020
1000 Objekt bearb. Wed Oct 17 12:04:55 CEST 2018
1000 Vgl. frl:6410553
1000 Oai Id
  1. oai:frl.publisso.de:frl:6410553 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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