1000 Titel
  • The Specific Monomer/Dimer Equilibrium of the Corticotropin-releasing Factor Receptor Type 1 Is Established in the Endoplasmic Reticulum
1000 Autor/in
  1. Teichmann, Anke |
  2. Gibert, Arthur |
  3. Lampe, André |
  4. Grzesik, Paul |
  5. Rutz, Claudia |
  6. Furkert, Jens |
  7. Schmoranzer, Jan |
  8. Krause, Gerd |
  9. Wiesner, Burkhard |
  10. Schülein, Ralf |
1000 Erscheinungsjahr 2014
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-06-25
1000 Erschienen in
1000 Quellenangabe
  • 289: 24250-24262
1000 FRL-Sammlung
1000 Verlagsversion
  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148855/ |
  • http://doi.org/10.1074/jbc.M114.553644 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • G protein-coupled receptors (GPCRs) represent the most important drug targets. Although the smallest functional unit of a GPCR is a monomer, it became clear in the past decades that the vast majority of the receptors form dimers. Only very recently, however, data were presented that some receptors may in fact be expressed as a mixture of monomers and dimers and that the interaction of the receptor protomers is dynamic. To date, equilibrium measurements were restricted to the plasma membrane due to experimental limitations. We have addressed the question as to where this equilibrium is established for the corticotropin-releasing factor receptor type 1. By developing a novel approach to analyze single molecule fluorescence cross-correlation spectroscopy data for intracellular membrane compartments, we show that the corticotropin-releasing factor receptor type 1 has a specific monomer/dimer equilibrium that is already established in the endoplasmic reticulum (ER). It remains constant at the plasma membrane even following receptor activation. Moreover, we demonstrate for seven additional GPCRs that they are expressed in specific but substantially different monomer/dimer ratios. Although it is well known that proteins may dimerize in the ER in principle, our data show that the ER is also able to establish the specific monomer/dimer ratios of GPCRs, which sheds new light on the functions of this compartment.
1000 Sacherschließung
lokal Endoplasmic Reticulum (ER)
lokal Monomer/Oligomer Equilibrium
lokal Plasma Membrane
lokal G Protein-coupled Receptor (GPCR)
lokal Corticotropin-releasing Factor Receptors
lokal Confocal Microscopy
lokal Fluorescence Cross-correlation Spectroscopy
lokal Receptor Oligomerization
lokal Fluorescence Correlation Spectroscopy (FCS)
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/VGVpY2htYW5uLMKgQW5rZQ==|https://frl.publisso.de/adhoc/creator/R2liZXJ0LMKgQXJ0aHVy|https://frl.publisso.de/adhoc/creator/TGFtcGUswqBBbmRyw6k=|https://frl.publisso.de/adhoc/creator/R3J6ZXNpayzCoFBhdWw=|https://frl.publisso.de/adhoc/creator/UnV0eiwgQ2xhdWRpYQ==|https://frl.publisso.de/adhoc/creator/RnVya2VydCzCoEplbnM=|https://frl.publisso.de/adhoc/creator/U2NobW9yYW56ZXIswqBKYW4=|https://frl.publisso.de/adhoc/creator/S3JhdXNlLMKgR2VyZA==|https://frl.publisso.de/adhoc/creator/V2llc25lciwgQnVya2hhcmQ=|https://frl.publisso.de/adhoc/creator/U2Now7xsZWluLCBSYWxm
1000 Label
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402327.rdf
1000 Erstellt am 2017-05-09T14:56:45.548+0200
1000 Erstellt von 25
1000 beschreibt frl:6402327
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Aug 18 08:03:46 CEST 2022
1000 Objekt bearb. Mon Dec 07 17:23:07 CET 2020
1000 Vgl. frl:6402327
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402327 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source