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WeightNameValue
1000 Titel
  • Depletion of highly abundant proteins from human cerebrospinal fluid: a cautionary note
1000 Autor/in
  1. Günther, Ramona |
  2. Krause, Eberhard |
  3. Schümann, Michael |
  4. Blasig, Ingolf E. |
  5. Haseloff, Reiner F. |
1000 Erscheinungsjahr 2015
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-10-15
1000 Erschienen in
1000 Quellenangabe
  • 10: 53
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2015
1000 Lizenz
1000 Verlagsversion
  • http://doi.org/10.1186/s13024-015-0050-7 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608131/ |
1000 Ergänzendes Material
  • https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-015-0050-7#Declarations |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Affinity-based techniques, both for enrichment or depletion of proteins of interest, suffer from unwanted interactions between the bait or matrix material and molecules different from the original target. This effect was quantitatively studied by applying two common procedures for the depletion of albumin/gamma immunoglobulin to human cerebrospinal fluid. Proteins of the depleted and the column-bound fraction were identified by mass spectrometry, employing 18O labeling for quantitation of their abundance. To different extents, the depletion procedures caused the loss of proteins previously suggested as biomarker candidates for neurological diseases. This is an important phenomenon to consider when quantifying protein levels in biological fluids.
1000 Sacherschließung
lokal Biomarker
lokal Unspecific binding
lokal Quantitation
lokal Albumin depletion
lokal Mass spectrometry
lokal Human cerebrospinal fluid
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R8O8bnRoZXIsIFJhbW9uYQ==|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBFYmVyaGFyZA==|https://frl.publisso.de/adhoc/creator/U2Now7xtYW5uLCBNaWNoYWVs|https://frl.publisso.de/adhoc/creator/Qmxhc2lnLCBJbmdvbGbCoEUu|https://frl.publisso.de/adhoc/creator/SGFzZWxvZmYsIFJlaW5lcsKgRi4=
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