WeightNameValue
1000 Titel
  • Routes of Epithelial Water Flow: Aquaporins versus Cotransporters
1000 Autor/in
  1. Mollajew, Rustam |
  2. Zocher, Florian |
  3. Horner, Andreas |
  4. Wiesner, Burkhard |
  5. Klussmann, Enno |
  6. Pohl, Peter |
1000 Erscheinungsjahr 2010
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2010-12-01
1000 Erschienen in
1000 Quellenangabe
  • 99(11): 3647–3656
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998630/ |
  • http://dx.doi.org/10.1016/j.bpj.2010.10.021 |
1000 Ergänzendes Material
  • http://www.cell.com/action/showImagesData?pii=S0006-3495%2810%2901285-3 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The routes water takes through membrane barriers is still a matter of debate. Although aquaporins only allow transmembrane water movement along an osmotic gradient, cotransporters are believed to be capable of water transport against the osmotic gradient. Here we show that the renal potassium-chloride-cotransporter (KCC1) does not pump a fixed amount of water molecules per movement of one K+ and one Cl−, as was reported for the analogous transporter in the choroid plexus. We monitored water and potassium fluxes through monolayers of primary cultured renal epithelial cells by detecting tiny solute concentration changes in the immediate vicinity of the monolayer. KCC1 extruded K+ ions in the presence of a transepithelial K+ gradient, but did not transport water. KCC1 inhibition reduced epithelial osmotic water permeability Pf by roughly one-third, i.e., the effect of inhibitors was small in resting cells and substantial in hormonal stimulated cells that contained high concentrations of aquaporin-2 in their apical membranes. The furosemide or DIOA (dihydroindenyl-oxy-alkanoic acid)-sensitive water flux was much larger than expected when water passively followed the KCC1-mediated ion flow. The inhibitory effect of these drugs on water flux was reversed by the K+–H+ exchanger nigericin, indicating that KCC1 affects water transport solely by K+ extrusion. Intracellular K+ retention conceivably leads to cell swelling, followed by an increased rate of endocytic AQP2 retrieval from the apical membrane.
1000 Fachgruppe
  1. Medizin |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TW9sbGFqZXcsIFJ1c3RhbQ==|https://frl.publisso.de/adhoc/creator/Wm9jaGVyLCBGbG9yaWFu|https://frl.publisso.de/adhoc/creator/SG9ybmVyLCBBbmRyZWFz|https://frl.publisso.de/adhoc/creator/V2llc25lciwgQnVya2hhcmQ=|https://frl.publisso.de/adhoc/creator/S2x1c3NtYW5uLCBFbm5v|http://orcid.org/0000-0002-1792-2314
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
1000 Fördernummer
  1. Po 533/8; Kl 1415/3-2
1000 Förderprogramm
  1. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer Po 533/8; Kl 1415/3-2
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402741.rdf
1000 Erstellt am 2017-06-02T09:46:02.934+0200
1000 Erstellt von 25
1000 beschreibt frl:6402741
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 15:29:58 CET 2020
1000 Objekt bearb. Wed Aug 01 12:39:25 CEST 2018
1000 Vgl. frl:6402741
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402741 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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