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WeightNameValue
1000 Titel
  • Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns
1000 Autor/in
  1. Szafranski, Karol |
  2. Schindler, Stefanie |
  3. Taudien, Stefan |
  4. Hiller, Michael |
  5. Huse, Klaus |
  6. Jahn, Niels |
  7. Schreiber, Stefan |
  8. Backofen, Rolf |
  9. Platzer, Matthias |
1000 Erscheinungsjahr 2007
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2007-08-01
1000 Erschienen in
1000 Quellenangabe
  • 8: R154
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2007
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1186/gb-2007-8-8-r154 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374985/ |
1000 Ergänzendes Material
  • http://genomebiology.biomedcentral.com/articles/10.1186/gb-2007-8-8-r154#Declarations |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Despite some degeneracy of sequence signals that govern splicing of eukaryotic pre-mRNAs, it is an accepted rule that U2-dependent introns exhibit the 3' terminal dinucleotide AG. Intrigued by anecdotal evidence for functional non-AG 3' splice sites, we carried out a human genome-wide screen. RESULTS: We identified TG dinucleotides functioning as alternative 3' splice sites in 36 human genes. The TG-derived splice variants were experimentally validated with a success rate of 92%. Interestingly, ratios of alternative splice variants are tissue-specific for several introns. TG splice sites and their flanking intron sequences are substantially conserved between orthologous vertebrate genes, even between human and frog, indicating functional relevance. Remarkably, TG splice sites are exclusively found as alternative 3' splice sites, never as the sole 3' splice site for an intron, and we observed a distance constraint for TG-AG splice site tandems. CONCLUSION: Since TGs splice sites are exclusively found as alternative 3' splice sites, the U2 spliceosome apparently accomplishes perfect specificity for 3' AGs at an early splicing step, but may choose 3' TGs during later steps. Given the tiny fraction of TG 3' splice sites compared to the vast amount of non-viable TGs, cis-acting sequence signals must significantly contribute to splice site definition. Thus, we consider TG-AG 3' splice site tandems as promising subjects for studies on the mechanisms of 3' splice site selection.
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U3phZnJhbnNraSwgS2Fyb2w=|https://frl.publisso.de/adhoc/creator/U2NoaW5kbGVyLCBTdGVmYW5pZQ==|https://frl.publisso.de/adhoc/creator/VGF1ZGllbiwgU3RlZmFu|https://frl.publisso.de/adhoc/creator/SGlsbGVyLCBNaWNoYWVs|http://orcid.org/0000-0003-3854-1884|https://frl.publisso.de/adhoc/creator/SmFobiwgTmllbHM=|https://frl.publisso.de/adhoc/creator/U2NocmVpYmVyLCBTdGVmYW4=|https://frl.publisso.de/adhoc/creator/QmFja29mZW4sIFJvbGY=|http://orcid.org/0000-0003-0596-8582
1000 Label
1000 Förderer
  1. German Ministry of Education and Research |
  2. Deutsche Forschungsgemeinschaft |
1000 Fördernummer
  1. 01GS0426; 01GR0504; 0313652D
  2. SFB604-02
1000 Förderprogramm
  1. -
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Ministry of Education and Research |
    1000 Förderprogramm -
    1000 Fördernummer 01GS0426; 01GR0504; 0313652D
  2. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SFB604-02
1000 Objektart article
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1000 @id frl:6402863.rdf
1000 Erstellt am 2017-06-07T08:36:03.309+0200
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