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1000 Titel
  • Three-dimensional mouse brain cytoarchitecture revealed by laboratory-based x-ray phase-contrast tomography
1000 Autor/in
  1. Töpperwien, Mareike |
  2. Krenkel, Martin |
  3. Vincenz, Daniel |
  4. Stöber, Franziska |
  5. Oelschlegel, Anja M. |
  6. Goldschmidt, Jürgen |
  7. Salditt, Tim |
1000 Erscheinungsjahr 2017
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-02-27
1000 Erschienen in
1000 Quellenangabe
  • 7:42847
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1038/srep42847 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5327439/ |
1000 Ergänzendes Material
  • http://www.nature.com/articles/srep42847#supplementary-information |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Studies of brain cytoarchitecture in mammals are routinely performed by serial sectioning of the specimen and staining of the sections. The procedure is labor-intensive and the 3D architecture can only be determined after aligning individual 2D sections, leading to a reconstructed volume with non-isotropic resolution. Propagation-based x-ray phase-contrast tomography offers a unique potential for high-resolution 3D imaging of intact biological specimen due to the high penetration depth and potential resolution. We here show that even compact laboratory CT at an optimized liquid-metal jet microfocus source combined with suitable phase-retrieval algorithms and a novel tissue preparation can provide cellular and subcellular resolution in millimeter sized samples of mouse brain. We removed water and lipids from entire mouse brains and measured the remaining dry tissue matrix in air, lowering absorption but increasing phase contrast. We present single-cell resolution images of mouse brain cytoarchitecture and show that axons can be revealed in myelinated fiber bundles. In contrast to optical 3D techniques our approach does neither require staining of cells nor tissue clearing, procedures that are increasingly difficult to apply with increasing sample and brain sizes. The approach thus opens a novel route for high-resolution high-throughput studies of brain architecture in mammals.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/IFTDtnBwZXJ3aWVuLCBNYXJlaWtl|https://frl.publisso.de/adhoc/creator/IEtyZW5rZWwsIE1hcnRpbg==|https://frl.publisso.de/adhoc/creator/IFZpbmNlbnosIERhbmllbA==|https://frl.publisso.de/adhoc/creator/IFN0w7ZiZXIsICBGcmFuemlza2E=|https://frl.publisso.de/adhoc/creator/IE9lbHNjaGxlZ2VsLCBBbmphIE0u|https://frl.publisso.de/adhoc/creator/IEdvbGRzY2htaWR0LCBKw7xyZ2Vu|https://frl.publisso.de/adhoc/creator/IFNhbGRpdHQsIFRpbQ==
1000 Förderer
  1. German science foundation (DFG) |
1000 Fördernummer
  1. 171; 755
1000 Förderprogramm
  1. Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain; Collaborative Research Center Nanoscale Photonic Imaging
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German science foundation (DFG) |
    1000 Förderprogramm Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain; Collaborative Research Center Nanoscale Photonic Imaging
    1000 Fördernummer 171; 755
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402885.rdf
1000 Erstellt am 2017-06-07T10:58:20.000+0200
1000 Erstellt von 122
1000 beschreibt frl:6402885
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Jun 27 11:16:50 CEST 2018
1000 Objekt bearb. Wed Jun 27 11:16:50 CEST 2018
1000 Vgl. frl:6402885
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402885 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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