v09p0907.pdf 843,43KB
1000 Titel
  • PBMC Transcription Profiles of Pigs with Divergent Humoral Immune Responses and Lean Growth Performance
1000 Autor/in
  1. Adler, Marcel |
  2. Murani, Eduard |
  3. Ponsuksili, Siriluck |
  4. Wimmers, Klaus |
1000 Erscheinungsjahr 2013
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2013-09-20
1000 Erschienen in
1000 Quellenangabe
  • 9(9): 907-916
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2013
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: The identification of key genes and regulatory networks in the transcriptomic responses of blood cells to antigen stimulation could facilitate the understanding of host defence and disease resistance. Moreover, genetic relationships between immunocompetence and the expression of other phenotypes, such as those of metabolic interest, are debated but incompletely understood in farm animals. Both positive and negative associations between immune responsiveness and performance traits such as weight gain or lean growth have been reported. We designed an in vivo microarray study of transcriptional changes in porcine peripheral blood mononuclear cells (PBMCs) during the immune response to tetanus toxoid (TT) as a model antigen for combined cellular (Th1) and humoral (Th2) responses. The aim of the study was to investigate the responsiveness of PBMCs against the background of divergent lean growth (LG) performance and anti-TT antibody (AB) titers and to compare lean growth and humoral immune performance phenotypes. RESULTS: In general, high LG phenotypes had increased cellular immune response transcripts, while low AB phenotypes had increased transcripts for canonical pathways that represented processes of intracellular and second messenger signaling and immune responses. Comparison of lean growth phenotypes in the context of high AB titers revealed higher cellular immune response transcripts in high LG phenotypes. Similar comparisons in the context of low AB titers failed to identify any corresponding pathways. When high and low AB titer phenotypes were differentially compared, low AB phenotypes had higher cellular immune response transcripts on a low LG background and higher cell signaling, growth, and proliferation transcripts on a high LG background. CONCLUSIONS: Divergent phenotypes of both lean growth performance and humoral immune response are affected by significant and functional transcript abundance changes throughout the immune response. The selected high-performance phenotypes demonstrated both high AB titers and increased transcript abundance of cellular immune response genes, which were possibly offset by lower expression of other cellular functions. Further, indications of compensatory effects were observed between cellular and humoral immune responses that became visible only in low-performance phenotypes.
1000 Sacherschließung
lokal microarray
lokal leukocyte
lokal porcine
lokal tetanus vaccination
lokal pathway analysis
1000 Fachgruppe
  1. Biologie |
  2. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Förderer
  1. German Federal Ministry of Education and Research |
1000 Fördernummer
  1. FKZ 0315130 A
1000 Förderprogramm
  1. GeneDialog project FUGATO plus
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Federal Ministry of Education and Research |
    1000 Förderprogramm GeneDialog project FUGATO plus
    1000 Fördernummer FKZ 0315130 A
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402897.rdf
1000 Erstellt am 2017-06-07T11:39:26.350+0200
1000 Erstellt von 23
1000 beschreibt frl:6402897
1000 Bearbeitet von 291
1000 Zuletzt bearbeitet Fri Jun 01 14:06:38 CEST 2018
1000 Objekt bearb. Fri Jun 01 14:06:38 CEST 2018
1000 Vgl. frl:6402897
1000 Oai Id
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1000 Sichtbarkeit Metadaten public
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