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1000 Titel
  • Gene Regulation of Intestinal Porcine Epithelial Cells IPEC-J2 Is Dependent on the Site of Deoxynivalenol Toxicological Action
1000 Autor/in
  1. Diesing, Anne-Kathrin |
  2. Nossol, Constanze |
  3. Ponsuksili, Siriluck |
  4. Kluess, Jeannette |
  5. Walk, Nicole |
  6. Post, Andreas |
  7. Rothkoetter, Hermann-Josef |
  8. Kahlert, Stefan |
  9. Wimmers, Klaus |
1000 Erscheinungsjahr 2012
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2012-04-10
1000 Erschienen in
1000 Quellenangabe
  • 7(4): e34136
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2012
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0034136 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323619/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The intestinal epithelial cell layer represents the border between the luminal and systemic side of the gut. The decision between absorption and exclusion of substances is the quintessential function of the gut and varies along the gut axis. Consequently, potentially toxic substances may reach the basolateral domain of the epithelial cell layer via blood stream. The mycotoxin deoxynivalenol (DON) is a Fusarium derived secondary metabolite known to enter the blood stream and displaying a striking toxicity on the basolateral side of polarised epithelial cell layers in vitro. Here we analysed potential mechanisms of apical and basolateral DON toxicity reflected in the gene expression. We used the jejunum-derived, polarised intestinal porcine epithelial cell line IPEC-J2 as an in vitro cell culture model. Luminal and systemic DON challenge of the epithelial cell layer was mimicked by a DON application from the apical or basolateral compartment of membrane inserts for 72 h. We compared the genome-wide gene expression of untreated and DON-treated IPEC-J2 cells with the GeneChip® Porcine Genome Array of Affymetrix. Low basolateral DON (200 ng/mL) application triggered 10 times more gene transcripts in comparison to the corresponding apical application (2539 versus 267) despite the intactness of the challenged cell layer as measured by transepithelial electrical resistance. Analysis of the regulated genes by bioinformatic resource DAVID identified several groups of biochemical pathways modulated by concentration and orientation of DON application. Selected genes representing pathways of the cellular metabolism, information processing and structural design were analysed in detail by quantitative PCR. Our findings clearly show that apical and basolateral challenge of epithelial cell layers trigger different gene response profiles paralleled with a higher susceptibility towards basolateral challenge. The evaluation of toxicological potentials of mycotoxins should take this difference in gene regulation dependent on route of application into account.
1000 Sacherschließung
lokal Epithelial cells
lokal Microarrays
lokal Gene regulation
lokal Cell metabolism
lokal Gastrointestinal tract
lokal Cell cultures
lokal Gene expression
lokal DNA-binding proteins
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/RGllc2luZywgQW5uZS1LYXRocmlu|https://frl.publisso.de/adhoc/creator/Tm9zc29sLCBDb25zdGFuemU=|https://frl.publisso.de/adhoc/creator/UG9uc3Vrc2lsaSwgU2lyaWx1Y2s=|https://frl.publisso.de/adhoc/creator/S2x1ZXNzLCBKZWFubmV0dGU=|https://frl.publisso.de/adhoc/creator/V2FsaywgTmljb2xl|https://frl.publisso.de/adhoc/creator/UG9zdCwgQW5kcmVhcw==|https://frl.publisso.de/adhoc/creator/Um90aGtvZXR0ZXIsIEhlcm1hbm4tSm9zZWY=|https://frl.publisso.de/adhoc/creator/S2FobGVydCwgU3RlZmFu|http://orcid.org/0000-0002-9523-6790
1000 Förderer
  1. European Union |
  2. Deutsche Forschungsgemeinschaft |
1000 Fördernummer
  1. grant agreement-no. 227549
  2. RO 743/3-2
1000 Förderprogramm
  1. “INTERPLAY”
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm “INTERPLAY”
    1000 Fördernummer grant agreement-no. 227549
  2. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer RO 743/3-2
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6402952.rdf
1000 Erstellt am 2017-06-08T12:04:13.936+0200
1000 Erstellt von 21
1000 beschreibt frl:6402952
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed May 16 12:50:51 CEST 2018
1000 Objekt bearb. Wed May 16 12:50:51 CEST 2018
1000 Vgl. frl:6402952
1000 Oai Id
  1. oai:frl.publisso.de:frl:6402952 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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