WeightNameValue
1000 Titel
  • MEK1 Binds Directly to βArrestin1, Influencing Both Its Phosphorylation by ERK and the Timing of Its Isoprenaline-stimulated Internalization
1000 Autor/in
  1. Meng, Dong |
  2. Lynch, Martin J. |
  3. Huston, Elaine |
  4. Beyermann, Michael |
  5. Eichhorst, Jenny |
  6. Adams, David R. |
  7. Klussmann, Enno |
  8. Houslay, Miles D. |
  9. Baillie, George S. |
1000 Erscheinungsjahr 2009
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2009-01-19
1000 Erschienen in
1000 Quellenangabe
  • 284: 11425-11435
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2670148/ |
  • http://doi.org/10.1074/jbc.M806395200 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • βArrestin is a multifunctional signal scaffold protein. Using SPOT immobilized peptide arrays, coupled with scanning alanine substitution and mutagenesis, we show that the MAPK kinase, MEK1, interacts directly with βarrestin1. Asp26 and Asp29 in the N-terminal domain of βarrestin1 are critical for its binding to MEK1, whereas Arg47 and Arg49 in the N-terminal domain of MEK1 are critical for its binding to βarrestin1. Wild-type FLAG-tagged βarrestin1 co-immunopurifies with MEK1 in HEKB2 cells, whereas the D26A/D29A mutant does not. ERK-dependent phosphorylation at Ser412 was compromised in the D26A/D29A-βarrestin1 mutant. A cell-permeable, 25-mer N-stearoylated βarrestin1 peptide that encompassed the N-domain MEK1 binding site blocked βarrestin1/MEK1 association in HEK cells and recapitulated the altered phenotype seen with the D26A/D29A-βarrestin1 in compromising the ERK-dependent phosphorylation of βarrestin1. In addition, the MEK disruptor peptide promoted the ability of βarrestin1 to co-immunoprecipitate with endogenous c-Src and clathrin, facilitating the isoprenaline-stimulated internalization of the β2-adrenergic receptor.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TWVuZywgRG9uZw==|https://frl.publisso.de/adhoc/creator/THluY2gsIE1hcnRpbiBKLg==|https://frl.publisso.de/adhoc/creator/SHVzdG9uLCBFbGFpbmU=|https://frl.publisso.de/adhoc/creator/QmV5ZXJtYW5uLCBNaWNoYWVs|https://frl.publisso.de/adhoc/creator/RWljaGhvcnN0LCBKZW5ueQ==|https://frl.publisso.de/adhoc/creator/QWRhbXMsIERhdmlkIFIu|https://frl.publisso.de/adhoc/creator/S2x1c3NtYW5uLCBFbm5v|https://frl.publisso.de/adhoc/creator/SG91c2xheSwgTWlsZXMgRC4=|https://frl.publisso.de/adhoc/creator/QmFpbGxpZSwgR2VvcmdlIFMu
1000 Förderer
  1. Medical Research Council Grants |
  2. European Union |
1000 Fördernummer
  1. G8604010; G0400053
  2. 037189
1000 Förderprogramm
  1. -
  2. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Medical Research Council Grants |
    1000 Förderprogramm -
    1000 Fördernummer G8604010; G0400053
  2. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm -
    1000 Fördernummer 037189
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403010.rdf
1000 Erstellt am 2017-06-13T11:00:08.931+0200
1000 Erstellt von 25
1000 beschreibt frl:6403010
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 28 11:18:08 CEST 2018
1000 Objekt bearb. Mon May 28 11:18:08 CEST 2018
1000 Vgl. frl:6403010
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403010 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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