PI3K Class II α Controls Spatially Restricted Endosomal PtdIns3P and Rab11 Activation to Promote Primary Cilium Function

  1. Franco, Irene
  2. Gulluni, Federico
  3. Campa, Carlo C.
  4. Costa, Carlotta
  5. Piero Margaria, Jean
  6. Ciraolo, Elisa
  7. Martini, Miriam
  8. Monteyne, Daniel
  9. De Luca, Elisa
  10. Germena, Giulia
  11. Posor, York
  12. Maffucci, Tania
  13. Marengo, Stefano
  14. Falasca, Marco
  15. Perez-Morga, David
  16. Boletta, Alessandra
  17. Merlo, Giorgio R.
  18. Hirsch, Emilio
  19. Haucke, Volker ORCID logo

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WeightNameValue
1000 Titel
  • PI3K Class II α Controls Spatially Restricted Endosomal PtdIns3P and Rab11 Activation to Promote Primary Cilium Function
1000 Autor/in
  1. Franco, Irene |
  2. Gulluni, Federico |
  3. Campa, Carlo C. |
  4. Costa, Carlotta |
  5. Piero Margaria, Jean |
  6. Ciraolo, Elisa |
  7. Martini, Miriam |
  8. Monteyne, Daniel |
  9. De Luca, Elisa |
  10. Germena, Giulia |
  11. Posor, York |
  12. Maffucci, Tania |
  13. Marengo, Stefano |
  14. Falasca, Marco |
  15. Perez-Morga, David |
  16. Boletta, Alessandra |
  17. Merlo, Giorgio R. |
  18. Hirsch, Emilio |
  19. Haucke, Volker |
1000 Erscheinungsjahr 2014
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-03-31
1000 Erschienen in
1000 Quellenangabe
  • 28(6): 647–658
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2014
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1016/j.devcel.2014.01.022 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4042153/ |
1000 Ergänzendes Material
  • http://www.cell.com/cms/attachment/2038221011/2052460616/mmc1.pdf |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Multiple phosphatidylinositol (PtdIns) 3-kinases (PI3Ks) can produce PtdIns3P to control endocytic trafficking, but whether enzyme specialization occurs in defined subcellular locations is unclear. Here, we report that PI3K-C2α is enriched in the pericentriolar recycling endocytic compartment (PRE) at the base of the primary cilium, where it regulates production of a specific pool of PtdIns3P. Loss of PI3K-C2α-derived PtdIns3P leads to mislocalization of PRE markers such as TfR and Rab11, reduces Rab11 activation, and blocks accumulation of Rab8 at the primary cilium. These changes in turn cause defects in primary cilium elongation, Smo ciliary translocation, and Sonic Hedgehog (Shh) signaling and ultimately impair embryonic development. Selective reconstitution of PtdIns3P levels in cells lacking PI3K-C2α rescues Rab11 activation, primary cilium length, and Shh pathway induction. Thus, PI3K-C2α regulates the formation of a PtdIns3P pool at the PRE required for Rab11 and Shh pathway activation.
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/RnJhbmNvLCBJcmVuZSA=|https://frl.publisso.de/adhoc/creator/R3VsbHVuaSwgRmVkZXJpY28g|https://frl.publisso.de/adhoc/creator/Q2FtcGEsIENhcmxvIEMuIA==|https://frl.publisso.de/adhoc/creator/Q29zdGEsIENhcmxvdHRhIA==|https://frl.publisso.de/adhoc/creator/UGllcm8gTWFyZ2FyaWEsIEplYW4g|https://frl.publisso.de/adhoc/creator/Q2lyYW9sbywgRWxpc2Eg|https://frl.publisso.de/adhoc/creator/TWFydGluaSwgTWlyaWFtIA==|https://frl.publisso.de/adhoc/creator/TW9udGV5bmUsIERhbmllbCA=|https://frl.publisso.de/adhoc/creator/RGUgTHVjYSwgRWxpc2Eg|https://frl.publisso.de/adhoc/creator/R2VybWVuYSwgR2l1bGlhIA==|https://frl.publisso.de/adhoc/creator/UG9zb3IsIFlvcmsg|https://frl.publisso.de/adhoc/creator/TWFmZnVjY2ksIFRhbmlhIA==|https://frl.publisso.de/adhoc/creator/TWFyZW5nbywgU3RlZmFubyA=|https://frl.publisso.de/adhoc/creator/RmFsYXNjYSwgTWFyY28g|https://frl.publisso.de/adhoc/creator/UGVyZXotTW9yZ2EsIERhdmlkIA==|https://frl.publisso.de/adhoc/creator/Qm9sZXR0YSwgQWxlc3NhbmRyYSA=|https://frl.publisso.de/adhoc/creator/TWVybG8sIEdpb3JnaW8gUi4g|https://frl.publisso.de/adhoc/creator/SGlyc2NoLCBFbWlsaW8g|http://orcid.org/0000-0003-3119-6993
1000 Label
1000 Förderer
  1. German Funding Agency (DFG) |
1000 Fördernummer
  1. SFB740/C08; SFB958/A07
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Funding Agency (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer SFB740/C08; SFB958/A07
1000 Objektart article
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1000 @id frl:6403036.rdf
1000 Erstellt am 2017-06-13T12:53:51.801+0200
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1000 Objekt bearb. Thu Mar 04 08:17:24 CET 2021
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  1. oai:frl.publisso.de:frl:6403036 |
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