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1000 Titel
  • TLR ligands, but not modulators of histone modifiers, can induce the complex immune response pattern of endotoxin tolerance in mammary epithelial cells
1000 Autor/in
  1. Günther, Juliane |
  2. Petzl, Wolfram |
  3. Zerbe, Holm |
  4. Schuberth, Hans-Joachim |
  5. Seyfert, Hans-Martin |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-12-01
1000 Erschienen in
1000 Quellenangabe
  • 23(2): 155-164
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1177/1753425916681076 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Excessive stimulation of the TLR4 axis through LPS reduces the expression of some cytokine genes in immune cells, while stimulating the expression of immune defense genes during a subsequent bacterial infection. This endotoxin tolerance (ET) is mediated via epigenetic mechanisms. Priming the udder of cows with LPS was shown to induce ET in mammary epithelial cells (MEC), thereby protecting the udder against reinfection for some time. Seeking alternatives to LPS priming we tried to elicit ET by priming MEC with either lipopeptide (Pam2CSK4) via the TLR2/6 axis or inhibitors of histone-modifying enzymes. Pre-incubation of MEC with Pam2CSK4 enhanced baseline and induced expression of bactericidal (β-defensin; SLPI) and membrane protecting factors (SAA3, TGM3), while reducing the expression of cytokine- and chemokine-encoding genes (TNF, IL1β) after a subsequent pathogen challenge, the latter, however, not as efficiently as after LPS priming. Pre-treating MEC with various inhibitors of histone H3 modifiers (for demethylation, acetylation or deacetylation) all failed to induce any of the protective factors and only resulted in some dampening of cytokine gene expression after the re-challenge. Hence, triggering immune functions via the TLR axis, but not through those histone modifiers, induced the beneficial phenomenon of ET in MEC.
1000 Sacherschließung
lokal Endotoxin tolerance
lokal mammary epithelial cells
lokal mastitis
lokal epigenetic mechanisms
lokal immune modulation
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R8O8bnRoZXIsIEp1bGlhbmU=|https://frl.publisso.de/adhoc/creator/UGV0emwsIFdvbGZyYW0=|https://frl.publisso.de/adhoc/creator/WmVyYmUsIEhvbG0=|https://frl.publisso.de/adhoc/creator/U2NodWJlcnRoLCBIYW5zLUpvYWNoaW0=|https://frl.publisso.de/adhoc/creator/U2V5ZmVydCwgSGFucy1NYXJ0aW4=
1000 Förderer
  1. Zoëtis |
  2. Deutsche Forschungsgemeinschaft (DFG) |
1000 Fördernummer
  1. -
  2. GU 1487/1-1
1000 Förderprogramm
  1. -
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Zoëtis |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer GU 1487/1-1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403072.rdf
1000 Erstellt am 2017-06-14T12:46:09.045+0200
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1000 Zuletzt bearbeitet Wed Jun 27 11:17:26 CEST 2018
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