art:10.1186/s12864-016-3299-5.pdf 5,83MB
1000 Titel
  • Comparative genomics to explore phylogenetic relationship, cryptic sexual potential and host specificity of Rhynchosporium species on grasses
1000 Autor/in
  1. Penselin, Daniel |
  2. Münsterkötter, Martin |
  3. Kirsten, Susanne |
  4. Felder, Marius |
  5. Taudien, Stefan |
  6. Platzer, Matthias |
  7. Ashelford, Kevin |
  8. Paskiewicz, Konrad H |
  9. Harrison, Richard J. |
  10. Hughes, David J. |
  11. Wolf, Thomas |
  12. Shelest, Ekaterina |
  13. Graap, Jenny |
  14. Hoffmann, Jan |
  15. Wenzel, Claudia |
  16. Wöltje, Nadine |
  17. King, Kevin M. |
  18. Fitt, Bruce D. L. |
  19. Güldener, Ulrich |
  20. Avrova, Anna |
  21. Knogge, Wolfgang |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-11-22
1000 Erschienen in
1000 Quellenangabe
  • 17:953
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: The Rhynchosporium species complex consists of hemibiotrophic fungal pathogens specialized to different sweet grass species including the cereal crops barley and rye. A sexual stage has not been described, but several lines of evidence suggest the occurrence of sexual reproduction. Therefore, a comparative genomics approach was carried out to disclose the evolutionary relationship of the species and to identify genes demonstrating the potential for a sexual cycle. Furthermore, due to the evolutionary very young age of the five species currently known, this genus appears to be well-suited to address the question at the molecular level of how pathogenic fungi adapt to their hosts. RESULTS: The genomes of the different Rhynchosporium species were sequenced, assembled and annotated using ab initio gene predictors trained on several fungal genomes as well as on Rhynchosporium expressed sequence tags. Structures of the rDNA regions and genome-wide single nucleotide polymorphisms provided a hypothesis for intra-genus evolution. Homology screening detected core meiotic genes along with most genes crucial for sexual recombination in ascomycete fungi. In addition, a large number of cell wall-degrading enzymes that is characteristic for hemibiotrophic and necrotrophic fungi infecting monocotyledonous hosts were found. Furthermore, the Rhynchosporium genomes carry a repertoire of genes coding for polyketide synthases and non-ribosomal peptide synthetases. Several of these genes are missing from the genome of the closest sequenced relative, the poplar pathogen Marssonina brunnea, and are possibly involved in adaptation to the grass hosts. Most importantly, six species-specific genes coding for protein effectors were identified in R. commune. Their deletion yielded mutants that grew more vigorously in planta than the wild type. CONCLUSION: Both cryptic sexuality and secondary metabolites may have contributed to host adaptation. Most importantly, however, the growth-retarding activity of the species-specific effectors suggests that host adaptation of R. commune aims at extending the biotrophic stage at the expense of the necrotrophic stage of pathogenesis. Like other apoplastic fungi Rhynchosporium colonizes the intercellular matrix of host leaves relatively slowly without causing symptoms, reminiscent of the development of endophytic fungi. Rhynchosporium may therefore become an object for studying the mutualism-parasitism transition.
1000 Sacherschließung
lokal Sex-related genes
lokal Non-ribosomal peptide synthetases
lokal Leotiomycetes
lokal Whole genome sequencing
lokal CAZymes
lokal Polyketide synthases
lokal Rhynchosporium
lokal Host specificity
lokal Phylogenetic evolution
lokal Effectors
1000 Fachgruppe
  1. Biologie |
  2. Umweltwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. Leibniz Association |
  2. Scottish Government Rural and Environment Science and Analytical Services (RESAS) |
1000 Fördernummer
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  2. -
1000 Förderprogramm
  1. Competition Committee’s competitive procedure
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Leibniz Association |
    1000 Förderprogramm Competition Committee’s competitive procedure
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Scottish Government Rural and Environment Science and Analytical Services (RESAS) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
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1000 Erstellt am 2017-06-16T12:26:16.123+0200
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