WeightNameValue
1000 Titel
  • Dynamics of the Ligand Binding Domain Layer during AMPA Receptor Activation
1000 Autor/in
  1. Baranovic, Jelena |
  2. Chebli, Miriam |
  3. Salazar, Hector |
  4. Carbone, Anna L. |
  5. Faelber, Katja |
  6. Lau, Albert Y. |
  7. Daumke, Oliver |
  8. Plested, Andrew |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-02-23
1000 Erschienen in
1000 Quellenangabe
  • 110(4): 896–911
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776031/ |
  • https://dx.doi.org/10.1016/j.bpj.2015.12.033 |
1000 Ergänzendes Material
  • http://www.biophysj.org/biophysj/supplemental/S0006-3495(16)00006-0 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Ionotropic glutamate receptors are postsynaptic tetrameric ligand-gated channels whose activity mediates fast excitatory transmission. Glutamate binding to clamshell-shaped ligand binding domains (LBDs) triggers opening of the integral ion channel, but how the four LBDs orchestrate receptor activation is unknown. Here, we present a high-resolution x-ray crystal structure displaying two tetrameric LBD arrangements fully bound to glutamate. Using a series of engineered metal ion trapping mutants, we showed that the more compact of the two assemblies corresponds to an arrangement populated during activation of full-length receptors. State-dependent cross-linking of the mutants identified zinc bridges between the canonical active LBD dimers that formed when the tetramer was either fully or partially bound by glutamate. These bridges also stabilized the resting state, consistent with the recently published full-length apo structure. Our results provide insight into the activation mechanism of glutamate receptors and the complex conformational space that the LBD layer can sample.
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/QmFyYW5vdmljLCBKZWxlbmE=|https://frl.publisso.de/adhoc/creator/Q2hlYmxpLCBNaXJpYW0=|https://frl.publisso.de/adhoc/creator/U2FsYXphciwgSGVjdG9y|https://frl.publisso.de/adhoc/creator/Q2FyYm9uZSwgQW5uYSBMLg==|https://frl.publisso.de/adhoc/creator/RmFlbGJlciwgS2F0amE=|https://frl.publisso.de/adhoc/creator/TGF1LCBBbGJlcnQgWS4=|https://frl.publisso.de/adhoc/creator/RGF1bWtlLCBPbGl2ZXI=|http://orcid.org/0000-0001-6062-0832
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
  2. Human Frontier Science Program (HFSP) |
1000 Fördernummer
  1. PL619.1; EXC-257
  2. -
1000 Förderprogramm
  1. Cluster of Excellence NeuroCure
  2. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm Cluster of Excellence NeuroCure
    1000 Fördernummer PL619.1; EXC-257
  2. 1000 joinedFunding-child
    1000 Förderer Human Frontier Science Program (HFSP) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403126.rdf
1000 Erstellt am 2017-06-16T14:21:24.758+0200
1000 Erstellt von 22
1000 beschreibt frl:6403126
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Thu Aug 18 07:58:57 CEST 2022
1000 Objekt bearb. Tue Mar 30 11:07:12 CEST 2021
1000 Vgl. frl:6403126
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403126 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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