WeightNameValue
1000 Titel
  • CIN85 Interacting Proteins in B Cells-Specific Role for SHIP-1
1000 Autor/in
  1. Büchse, Tom |
  2. Horras, Nikolaus |
  3. Lenfert, Eva |
  4. Krystal, Gerald |
  5. Körbel, Sandra |
  6. Schümann, Michael |
  7. Krause, Eberhard |
  8. Mikkat, Stefan |
  9. Tiedge, Markus |
1000 Erscheinungsjahr 2011
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2011-07-01
1000 Erschienen in
1000 Quellenangabe
  • 10: M110.006239
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205855/ |
  • http://doi.org/10.1074/mcp.M110.006239 |
1000 Ergänzendes Material
  • http://www.mcponline.org/content/10/10/M110.006239/suppl/DC1 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The Cbl-interacting 85-kDa protein (CIN85) plays an important role as a negative regulator of signaling pathways induced by receptor tyrosine kinases. By assembling multiprotein complexes this versatile adaptor enhances receptor tyrosine kinase-activated clathrin-mediated endocytosis and reduces phosphatidylinositol-3-kinase-induced phosphatidylinositol-3,4,5-trisphosphate production. Here we report the expression of CIN85 in primary splenic B lymphocytes and the B-lymphoma cell lines WEHI 231 and Ba/F3. Cross-linking of the B cell antigen receptor resulted in an increased association of CIN85 with the ubiquitin ligase Cbl. Through a systematic pull-down proteomics approach we identified 51 proteins that interact with CIN85 in B cells, including proteins not shown previously to be CIN85-associated. Among these proteins, the SH2-containing inositol phosphatase 1 (SHIP-1) co-precipitated with both the full-length CIN85 and each of its three SH3 domains. We also showed that this association is constitutive and depends on a region of 79 amino acids near the carboxyl terminus of SHIP-1, a region rich in potential SH3 domain binding sites. Because SHIP-1 is a major negative regulator of the phosphatidylinositol-3-kinase pathway in lymphocytes, we hypothesize that the interaction between SHIP-1 and CIN85 might synergistically facilitate the down-regulation of phosphatidylinositol-3,4,5-trisphosphate levels.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/QsO8Y2hzZSwgVG9t|https://frl.publisso.de/adhoc/creator/SG9ycmFzLCBOaWtvbGF1cw==|https://frl.publisso.de/adhoc/creator/TGVuZmVydCwgRXZh|https://frl.publisso.de/adhoc/creator/S3J5c3RhbCwgR2VyYWxk|https://frl.publisso.de/adhoc/creator/S8O2cmJlbCwgU2FuZHJh|https://frl.publisso.de/adhoc/creator/U2Now7xtYW5uLCBNaWNoYWVs|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBFYmVyaGFyZA==|https://frl.publisso.de/adhoc/creator/TWlra2F0LCBTdGVmYW4=|https://frl.publisso.de/adhoc/creator/VGllZGdlLCBNYXJrdXM=
1000 Objektart article
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1000 Erstellt am 2017-06-19T09:22:11.710+0200
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1000 Bearbeitet von 218
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1000 Vgl. frl:6403136
1000 Oai Id
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