WeightNameValue
1000 Titel
  • Regulation of synaptic vesicle recycling by complex formation between intersectin 1 and the clathrin adaptor complex AP2
1000 Autor/in
  1. Pechstein, Arndt |
  2. Bacetic, Jelena |
  3. Vahedi-Faridi, Ardeschir |
  4. Gromova, Kira |
  5. Sundborger, Anna |
  6. Tomlin, Nikolay |
  7. Krainer, Georg |
  8. Vorontsova, Olga |
  9. Schäfer, Johannes G. |
  10. Owe, Simen G. |
  11. Cousin, Michael A. |
  12. Saenger, Wolfram |
  13. Shupliakov, Oleg |
  14. Haucke, Volker |
1000 Erscheinungsjahr 2010
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2010-03-02
1000 Erschienen in
1000 Quellenangabe
  • 107(9): 4206–4211
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840162/ |
  • http://doi.org/10.1073/pnas.0911073107 |
1000 Ergänzendes Material
  • http://www.pnas.org/cgi/content/full/0911073107/DCSupplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Clathrin-mediated synaptic vesicle (SV) recycling involves the spatiotemporally controlled assembly of clathrin coat components at phosphatidylinositiol (4, 5)-bisphosphate [PI(4,5)P2]-enriched membrane sites within the periactive zone. Such spatiotemporal control is needed to coordinate SV cargo sorting with clathrin/AP2 recruitment and to restrain membrane fission and synaptojanin-mediated uncoating until membrane deformation and clathrin coat assembly are completed. The molecular events underlying these control mechanisms are unknown. Here we show that the endocytic SH3 domain-containing accessory protein intersectin 1 scaffolds the endocytic process by directly associating with the clathrin adaptor AP2. Acute perturbation of the intersectin 1-AP2 interaction in lamprey synapses in situ inhibits the onset of SV recycling. Structurally, complex formation can be attributed to the direct association of hydrophobic peptides within the intersectin 1 SH3A-B linker region with the “side sites” of the AP2 α- and β-appendage domains. AP2 appendage association of the SH3A-B linker region inhibits binding of the inositol phosphatase synaptojanin 1 to intersectin 1. These data identify the intersectin-AP2 complex as an important regulator of clathrin-mediated SV recycling in synapses.
1000 Sacherschließung
lokal appendage
lokal endocytosis
lokal scaffolding proteins
lokal synapse
lokal synaptojanin
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/UGVjaHN0ZWluLCBBcm5kdA==|https://frl.publisso.de/adhoc/creator/QmFjZXRpYywgSmVsZW5h|https://frl.publisso.de/adhoc/creator/VmFoZWRpLUZhcmlkaSwgQXJkZXNjaGly|https://frl.publisso.de/adhoc/creator/R3JvbW92YSwgS2lyYQ==|https://frl.publisso.de/adhoc/creator/U3VuZGJvcmdlciwgQW5uYQ==|https://frl.publisso.de/adhoc/creator/VG9tbGluLCBOaWtvbGF5|https://frl.publisso.de/adhoc/creator/S3JhaW5lciwgR2Vvcmc=|https://frl.publisso.de/adhoc/creator/Vm9yb250c292YSwgT2xnYQ==|https://frl.publisso.de/adhoc/creator/U2Now6RmZXIsIEpvaGFubmVzIEcu|https://frl.publisso.de/adhoc/creator/T3dlLCBTaW1lbiBHLg==|https://frl.publisso.de/adhoc/creator/Q291c2luLCBNaWNoYWVsIEEu|https://frl.publisso.de/adhoc/creator/U2FlbmdlciwgV29sZnJhbQ==|https://frl.publisso.de/adhoc/creator/U2h1cGxpYWtvdiwgT2xlZw==|http://orcid.org/0000-0003-3119-6993
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Swedish Research Council |
  3. Wallenbergs Foundation and Linné Center DBRM |
  4. European Science Foundation |
1000 Fördernummer
  1. HA2686/ 3-1/ FOR 806; GRK 1123
  2. 13473; 20587
  3. SFB 449/ Z3
  4. -
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer HA2686/ 3-1/ FOR 806; GRK 1123
  2. 1000 joinedFunding-child
    1000 Förderer Swedish Research Council |
    1000 Förderprogramm -
    1000 Fördernummer 13473; 20587
  3. 1000 joinedFunding-child
    1000 Förderer Wallenbergs Foundation and Linné Center DBRM |
    1000 Förderprogramm -
    1000 Fördernummer SFB 449/ Z3
  4. 1000 joinedFunding-child
    1000 Förderer European Science Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403194.rdf
1000 Erstellt am 2017-06-20T14:53:20.143+0200
1000 Erstellt von 25
1000 beschreibt frl:6403194
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 23:04:25 CET 2020
1000 Objekt bearb. Mon Jun 18 12:49:38 CEST 2018
1000 Vgl. frl:6403194
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403194 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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