journal.pone.0162485.PDF 2,19MB
1000 Titel
  • Transient Shifts of Incubation Temperature Reveal Immediate and Long-Term Transcriptional Response in Chicken Breast Muscle Underpinning Resilience and Phenotypic Plasticity
1000 Autor/in
  1. Naraballobh, Watcharapong |
  2. Trakooljul, Nares |
  3. Murani, Eduard |
  4. Brunner, Ronald |
  5. Krischek, Carsten |
  6. Janisch, Sabine |
  7. Wicke, Michael |
  8. Ponsuksili, Siriluck |
  9. Wimmers, Klaus |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-09-09
1000 Erschienen in
1000 Quellenangabe
  • 11(9): e0162485
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Variations in egg incubation temperatures can have acute or long-term effects on gene transcription in avian species. Altered gene expression may, in turn, affect muscle traits in poultry and indirectly influence commercial production. To determine how changes in eggshell temperature affect gene expression, incubation temperatures were varied [36.8°C (low), 37.8°C (control), 38.8°C (high)] at specific time periods reflecting two stages of myogenesis [embryonic days (ED) 7–10 and 10–13]. Gene expression was compared between interventions and matching controls by microarrays in broiler breast muscle at ED10 or ED13 and post-hatch at day 35. Early (ED7-10) high incubation temperature (H10ΔC) resulted in 1370 differentially expressed genes (DEGs) in embryos. Ingenuity pathway analysis revealed temporary activation of cell maintenance, organismal development, and survival ability genes, but these effects were not maintained in adults. Late high incubation temperature (ED10-13) (H13ΔC) had slightly negative impacts on development of cellular components in embryos, but a cumulative effect was observed in adults, in which tissue development and nutrition metabolism were affected. Early low incubation temperature (L10ΔC) produced 368 DEGs, most of which were down-regulated and involved in differentiation and formation of muscle cells. In adults, this treatment down-regulated pathways of transcriptional processes, but up-regulated cell proliferation. Late low temperature incubation (L13ΔC) produced 795 DEGs in embryos, and activated organismal survival and post-transcriptional regulation pathways. In adults this treatment activated cellular and organ development, nutrition and small molecule activity, and survival rate, but deactivated size of body and muscle cells. Thermal interventions during incubation initiate immediate and delayed transcriptional responses that are specific for timing and direction of treatment. Interestingly, the transcriptional response to transiently decreased incubation temperature, which did not affect the phenotypes, prompts compensatory effects reflecting resilience. In contrast, higher incubation temperature triggers gene expression and has long-term effects on the phenotype. These mechanisms of considerable phenotypic plasticity contribute to the biodiversity and broaden the basis for managing poultry populations.
1000 Sacherschließung
lokal Chickens
lokal Gene expression
lokal Genetic networks
lokal Body weight
lokal DNA transcription
lokal Body temperature
lokal Poultry
lokal Embryos
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. German Research Foundation (DFG) |
1000 Fördernummer
  1. WE 4079/4-1; WI 1754/15-1; WI 2072/9-1
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer WE 4079/4-1; WI 1754/15-1; WI 2072/9-1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403281.rdf
1000 Erstellt am 2017-06-28T12:31:48.313+0200
1000 Erstellt von 218
1000 beschreibt frl:6403281
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Tue Mar 30 11:18:16 CEST 2021
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