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1000 Titel
  • ST2 from rainbow trout quenches TLR signalling, localises at the nuclear membrane and allows the nuclear translocation of MYD88
1000 Autor/in
  1. Rebl, Henrike |
  2. Köbis, Judith M. |
  3. Seyfert, Hans-Martin |
  4. Rebl, Alexander |
  5. Goldammer, Tom |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-10-21
1000 Erschienen in
1000 Quellenangabe
  • 67: 139-152
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1016/j.dci.2016.10.009 |
1000 Ergänzendes Material
  • http://www.sciencedirect.com/science/article/pii/S0145305X16303585#appd001 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The mammalian interleukin 1 receptor-like 1 receptor (IL1RL1), commonly known as ST2, is thought to downregulate TLR signalling by sequestering the signalling adapter MYD88 (myeloid differentiation primary response protein 88). ST2 sequences are known in several fish species, but none of them have functionally been examined. We characterised ST2 from rainbow trout (Oncorhynchus mykiss) and the structure of its encoding gene. The primary sequence of ST2 is only weakly conserved from fish to human. However, the amino acid sequences forming the interfaces for ST2 and MYD88 interaction are well conserved throughout evolution. High similarity of the gene segmentation unambiguously proves the common ancestry of fish and mammalian ST2. Trout ST2 and trout MYD88 genes were constitutively expressed in embryonic, larval and adult trout. In vivo infection with Aeromonas salmonicida did not modulate the mRNA levels of both factors. Overexpressing trout ST2 in the mammalian HEK-293 reconstitution system of TLR2 signalling quenched the Escherichia coli–induced activation of NF-κB and SAA promoters in a dose-dependent fashion. The expression of GFP-tagged trout ST2 in human HEK-293 or trout CHSE-214 cells surprisingly revealed that (i) ST2 localised abundantly at the nuclear membrane rather than at the cell membrane and (ii) the coexpression of both ST2 and MYD88 allowed the translocation of trout MYD88 from cytoplasm to nucleus, as assessed using confocal microscopy and Western blotting. Hence, we validated that trout ST2 is a dampener of TLR signalling and interacts with MYD88. The spatial distribution of these factors raises questions about how this repressive mechanism functions.
1000 Sacherschließung
lokal Interleukin 1 receptor-like 1
lokal Toll-like receptor signalling
lokal HEK-293
lokal Evolution
lokal CHSE-214
lokal Oncorhynchus mykiss
lokal TIR-domain
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/UmVibCwgSGVucmlrZQ==|https://frl.publisso.de/adhoc/creator/S8O2YmlzLCBKdWRpdGggTS4=|https://frl.publisso.de/adhoc/creator/U2V5ZmVydCwgSGFucy1NYXJ0aW4=|http://orcid.org/0000-0002-2918-7433|http://orcid.org/0000-0003-1215-5504
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
  2. European Fisheries Fund (EFF) |
  3. Ministry of Agriculture’s, Environment and Consumer Protection Mecklenburg-Western Pomerania |
1000 Fördernummer
  1. SE 326/16-1
  2. -
  3. VI-560/7308-4
1000 Förderprogramm
  1. -
  2. -
  3. BORN trout
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer SE 326/16-1
  2. 1000 joinedFunding-child
    1000 Förderer European Fisheries Fund (EFF) |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Ministry of Agriculture’s, Environment and Consumer Protection Mecklenburg-Western Pomerania |
    1000 Förderprogramm BORN trout
    1000 Fördernummer VI-560/7308-4
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403418.rdf
1000 Erstellt am 2017-07-07T11:13:42.419+0200
1000 Erstellt von 122
1000 beschreibt frl:6403418
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Jun 20 12:52:10 CEST 2018
1000 Objekt bearb. Wed Jun 20 12:52:09 CEST 2018
1000 Vgl. frl:6403418
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403418 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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