Download
1-s2.0-S0044848616308845-main.pdf 730,67KB
WeightNameValue
1000 Titel
  • Microarray-predicted marker genes and molecular pathways indicating crowding stress in rainbow trout (Oncorhynchus mykiss)
1000 Autor/in
  1. Bochert, Ralf |
  2. Verleih, Marieke |
  3. Rebl, Alexander |
  4. Zebunke, Manuela |
  5. Borchel, Andreas |
  6. Goldammer, Tom |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-04-20
1000 Erschienen in
1000 Quellenangabe
  • 473:355-365
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1016/j.aquaculture.2017.03.003 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • STATEMENT OF RELEVANCE: The comprehensive recording of animal-based biomarkers is becoming increasingly important for the further development of the aquaculture industry with importance for fish well-being and consumer opinion. Previous studies on crowding stress in rainbow trout have used single or multi-gene approaches to assess the relevance of putative biomarkers, but transcriptomic approaches to define crowding stress-induced pathways in salmonids are overdue. The present study compares the transcriptomic profiles of rainbow trout exposed to different stocking densities and correlates these data sets with classical plasma parameters and behavioural observations.
  • Stocking density is considered as a key factor determining the productivity of fish aquaculture systems. The transcriptomic response to crowding stress is, however, still poorly investigated. We aimed to identify potential biomarker genes via microarray analyses to gain insight into molecular pathways modulated by density-induced stress in farmed rainbow trout Oncorhynchus mykiss. Transcriptome profiling in the liver, kidney, and gills was complemented by analysis of classical plasma parameters and behavioural observations. Individuals of two trout strains were exposed for nine days to definite stocking densities, ~ 10 kg/m3 (low density); ~ 30 kg/m3 (moderate); ~ 60 kg/m3 (elevated); and ~ 120 kg/m3 (high). Only the PYGMA gene, encoding the key enzyme of glycogenolysis, was upregulated in gills and kidney, while about 90 genes were differentially regulated in the liver of trout exposed to high density compared with the moderate-density group. Pathway enrichment analyses predicted the upregulation of HIF1A signalling in liver, contributing to glucose homeostasis during stress conditions. This is consistent with the increased plasma glucose levels detected in crowding-stressed trout. The mTOR and PI3K/AKT signalling pathways were impaired in liver at higher stocking densities indicating stress at the cellular level. Contrary to our expectations, protein ubiquitination and cholesterol biosynthesis pathways were found to be underexpressed. Cholesterol is a precursor of the stress hormone cortisol, whose plasma concentrations were indeed within the normal physiological range in all treatment groups. In addition, we did not observe any aggressive interactions, which are known to be triggered through cortisol release. Taken together, we identified a relatively modest number of rainbow trout-specific crowding markers suggesting that farmed rainbow trout have gained enhanced stress-tolerant phenotypes through decades of selective breeding.
1000 Sacherschließung
lokal Glucose metabolism
lokal Transcriptome
lokal Salmonid fish
lokal Welfare
lokal Glycogenolysis
lokal Stocking density
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/Qm9jaGVydCwgUmFsZg==|https://frl.publisso.de/adhoc/creator/VmVybGVpaCwgTWFyaWVrZQ==|http://orcid.org/0000-0002-2918-7433|http://orcid.org/0000-0002-5232-5873|http://orcid.org/0000-0002-1408-7261|http://orcid.org/0000-0003-1215-5504
1000 Label
1000 Förderer
  1. Ministry of Agriculture, the Environment and Consumer Protection Mecklenburg-Western Pomerania, Germany |
  2. Federal Ministry of Education and Research |
1000 Fördernummer
  1. VI-560/7308-4
  2. 0315536G
1000 Förderprogramm
  1. European Fisheries Fund (EFF); BORN-Forelle
  2. German agriculture network PHÄNOMICS
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Ministry of Agriculture, the Environment and Consumer Protection Mecklenburg-Western Pomerania, Germany |
    1000 Förderprogramm European Fisheries Fund (EFF); BORN-Forelle
    1000 Fördernummer VI-560/7308-4
  2. 1000 joinedFunding-child
    1000 Förderer Federal Ministry of Education and Research |
    1000 Förderprogramm German agriculture network PHÄNOMICS
    1000 Fördernummer 0315536G
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403454.rdf
1000 Erstellt am 2017-07-13T10:44:04.819+0200
1000 Erstellt von 25
1000 beschreibt frl:6403454
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Mon Jan 02 10:52:43 CET 2023
1000 Objekt bearb. Mon Jan 02 10:52:43 CET 2023
1000 Vgl. frl:6403454
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403454 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source