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WeightNameValue
1000 Titel
  • Benzylglucosinolate Derived Isothiocyanate from Tropaeolum majus Reduces Gluconeogenic Gene and Protein Expression in Human Cells
1000 Autor/in
  1. Guzmán-Pérez, Valentina |
  2. Bumke-Vogt, Christiane |
  3. Schreiner, Monika |
  4. Mewis, Inga |
  5. Borchert, Andrea |
  6. Pfeiffer, Andreas F. H. |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-09-13
1000 Erschienen in
1000 Quellenangabe
  • 11(9):e0162397
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1371/journal.pone.0162397 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021297/ |
1000 Ergänzendes Material
  • http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0162397#sec023 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Nasturtium (Tropaeolum majus L.) contains high concentrations of benzylglcosinolate. We found that a hydrolysis product of benzyl glucosinolate-the benzyl isothiocyanate (BITC)-modulates the intracellular localization of the transcription factor Forkhead box O 1 (FOXO1). FoxO transcription factors can antagonize insulin effects and trigger a variety of cellular processes involved in tumor suppression, longevity, development and metabolism. The current study evaluated the ability of BITC-extracted as intact glucosinolate from nasturtium and hydrolyzed with myrosinase-to modulate i) the insulin-signaling pathway, ii) the intracellular localization of FOXO1 and, iii) the expression of proteins involved in gluconeogenesis, antioxidant response and detoxification. Stably transfected human osteosarcoma cells (U-2 OS) with constitutive expression of FOXO1 protein labeled with GFP (green fluorescent protein) were used to evaluate the effect of BITC on FOXO1. Human hepatoma HepG2 cell cultures were selected to evaluate the effect on gluconeogenic, antioxidant and detoxification genes and protein expression. BITC reduced the phosphorylation of protein kinase B (AKT/PKB) and FOXO1; promoted FOXO1 translocation from cytoplasm into the nucleus antagonizing the insulin effect; was able to down-regulate the gene and protein expression of gluconeogenic enzymes; and induced the gene expression of antioxidant and detoxification enzymes. Knockdown analyses with specific siRNAs showed that the expression of gluconeogenic genes was dependent on nuclear factor (erythroid derived)-like2 (NRF2) and independent of FOXO1, AKT and NAD-dependent deacetylase sirtuin-1 (SIRT1). The current study provides evidence that BITC might have a role in type 2 diabetes T2D by reducing hepatic glucose production and increasing antioxidant resistance.
1000 Sacherschließung
lokal Antioxidants
lokal Oxidative stress
lokal Transcription factors
lokal Detoxification
lokal Small interfering RNAs
lokal Gene expression
lokal Insulin
lokal Forkhead box
1000 Fachgruppe
  1. Medizin |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R3V6bcOhbi1Qw6lyZXosIFZhbGVudGluYQ==|https://frl.publisso.de/adhoc/creator/QnVta2UtVm9ndCwgQ2hyaXN0aWFuZQ==|https://frl.publisso.de/adhoc/creator/U2NocmVpbmVyLCBNb25pa2E=|https://frl.publisso.de/adhoc/creator/TWV3aXMsIEluZ2E=|https://frl.publisso.de/adhoc/creator/Qm9yY2hlcnQsIEFuZHJlYQ==|https://frl.publisso.de/adhoc/creator/UGZlaWZmZXIsIEFuZHJlYXMgRi4gSC4=
1000 Förderer
  1. Investitionsbank Berlin (IBB) |
  2. German Academic Exchange Service (DAAD) |
  3. Pontificia Universidad Javeriana Bogota DC (Colombia) |
1000 Fördernummer
  1. 10140311
  2. A/09/72490
  3. -
1000 Förderprogramm
  1. Entwicklung einer biotechnologischen Methode zur Identifikation gesundheitlich vorteilhafter Pflanzeninhaltsstoffe zur Prävention von Diabetes Typ 2
  2. -
  3. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Investitionsbank Berlin (IBB) |
    1000 Förderprogramm Entwicklung einer biotechnologischen Methode zur Identifikation gesundheitlich vorteilhafter Pflanzeninhaltsstoffe zur Prävention von Diabetes Typ 2
    1000 Fördernummer 10140311
  2. 1000 joinedFunding-child
    1000 Förderer German Academic Exchange Service (DAAD) |
    1000 Förderprogramm -
    1000 Fördernummer A/09/72490
  3. 1000 joinedFunding-child
    1000 Förderer Pontificia Universidad Javeriana Bogota DC (Colombia) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403474.rdf
1000 Erstellt am 2017-07-14T11:14:09.867+0200
1000 Erstellt von 122
1000 beschreibt frl:6403474
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Fri May 25 07:20:55 CEST 2018
1000 Objekt bearb. Fri May 25 07:20:54 CEST 2018
1000 Vgl. frl:6403474
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403474 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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