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WeightNameValue
1000 Titel
  • Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission
1000 Autor/in
  1. Schöneberg, Johannes |
  2. Lehmann, Martin |
  3. Ullrich, Alexander |
  4. Posor, York |
  5. Lo, Wen-Ting |
  6. Schmoranzer, Jan |
  7. Noé, Frank |
  8. Lichtner, Gregor |
  9. Haucke, Volker |
1000 Erscheinungsjahr 2017
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-06-19
1000 Erschienen in
1000 Quellenangabe
  • 8: 15873
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • http://doi.org/10.1038/ncomms15873 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5481832/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/ncomms15873#supplementary-information |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Clathrin-mediated endocytosis (CME) involves membrane-associated scaffolds of the bin-amphiphysin-rvs (BAR) domain protein family as well as the GTPase dynamin, and is accompanied and perhaps triggered by changes in local lipid composition. How protein recruitment, scaffold assembly and membrane deformation is spatiotemporally controlled and coupled to fission is poorly understood. We show by computational modelling and super-resolution imaging that phosphatidylinositol 3,4-bisphosphate [PI(3,4)P2] synthesis within the clathrin-coated area of endocytic intermediates triggers selective recruitment of the PX-BAR domain protein SNX9, as a result of complex interactions of endocytic proteins competing for phospholipids. The specific architecture induces positioning of SNX9 at the invagination neck where its self-assembly regulates membrane constriction, thereby providing a template for dynamin fission. These data explain how lipid conversion at endocytic pits couples local membrane constriction to fission. Our work demonstrates how computational modelling and super-resolution imaging can be combined to unravel function and mechanisms of complex cellular processes.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U2Now7ZuZWJlcmcsIEpvaGFubmVz|https://frl.publisso.de/adhoc/creator/TGVobWFubizCoE1hcnRpbg==|https://frl.publisso.de/adhoc/creator/VWxscmljaCzCoEFsZXhhbmRlcg==|https://frl.publisso.de/adhoc/creator/UG9zb3IswqBZb3Jr|https://frl.publisso.de/adhoc/creator/TG8swqBXZW4tVGluZw==|https://frl.publisso.de/adhoc/creator/U2NobW9yYW56ZXIswqBKYW4=|https://frl.publisso.de/adhoc/creator/Tm-DqSwgRnJhbms=|http://orcid.org/0000-0002-5890-1958|http://orcid.org/0000-0003-3119-6993
1000 Förderer
  1. German Research Foundation |
  2. European Commission |
1000 Fördernummer
  1. SFB958/A04; SFB1114/C03; SFB958/Z02; SFB958/A07
  2. -
1000 Förderprogramm
  1. -
  2. ERC StG ‘pcCell’; Marie Skłodowska-Curie IOF
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation |
    1000 Förderprogramm -
    1000 Fördernummer SFB958/A04; SFB1114/C03; SFB958/Z02; SFB958/A07
  2. 1000 joinedFunding-child
    1000 Förderer European Commission |
    1000 Förderprogramm ERC StG ‘pcCell’; Marie Skłodowska-Curie IOF
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403543.rdf
1000 Erstellt am 2017-07-20T10:10:26.769+0200
1000 Erstellt von 25
1000 beschreibt frl:6403543
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 28 10:57:24 CEST 2018
1000 Objekt bearb. Mon May 28 10:57:24 CEST 2018
1000 Vgl. frl:6403543
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403543 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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