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WeightNameValue
1000 Titel
  • Exposure of Lactating Dairy Cows to Acute Pre-Ovulatory Heat Stress Affects Granulosa Cell-Specific Gene Expression Profiles in Dominant Follicles
1000 Autor/in
  1. Vernunft, Andreas |
  2. Koczan, Dirk |
  3. Spitschak, Marion |
  4. Kuhla, Björn |
  5. Vanselow, Jens |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-08-17
1000 Erschienen in
1000 Quellenangabe
  • 11(8):e0160600
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1371/journal.pone.0160600 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988698/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • High environmental temperatures induce detrimental effects on various reproductive processes in cattle. According to the predicted global warming the number of days with unfavorable ambient temperatures will further increase. The objective of this study was to investigate effects of acute heat stress during the late pre-ovulatory phase on morphological, physiological and molecular parameters of dominant follicles in cycling cows during lactation. Eight German Holstein cows in established lactation were exposed to heat stress (28°C) or thermoneutral conditions (15°C) with pair-feeding for four days. After hormonal heat induction growth of the respective dominant follicles was monitored by ultrasonography for two days, then an ovulatory GnRH dose was given and follicular steroid hormones and granulosa cell-specific gene expression profiles were determined 23 hrs thereafter. The data showed that the pre-ovulatory growth of dominant follicles and the estradiol, but not the progesterone concentrations tended to be slightly affected. mRNA microarray and hierarchical cluster analysis revealed distinct expression profiles in granulosa cells derived from heat stressed compared to pair-fed animals. Among the 255 affected genes heatstress-, stress- or apoptosis associated genes were not present. But instead, we found up-regulation of genes essentially involved in G-protein coupled signaling pathways, extracellular matrix composition, and several members of the solute carrier family as well as up-regulation of FST encoding follistatin. In summary, the data of the present study show that acute pre-ovulatory heat stress can specifically alter gene expression profiles in granulosa cells, however without inducing stress related genes and pathways and suggestively can impair follicular growth due to affecting the activin-inhibin-follistatin system.
1000 Sacherschließung
lokal Growth factors
lokal Oocytes
lokal Heat shock response
lokal Therrmal stresses
lokal Gene expression
lokal Microarrays
lokal Apoptosis
lokal Granulosa cells
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/VmVybnVuZnQsIEFuZHJlYXM=|https://frl.publisso.de/adhoc/creator/S29jemFuLCBEaXJr|https://frl.publisso.de/adhoc/creator/U3BpdHNjaGFrLCBNYXJpb24=|https://frl.publisso.de/adhoc/creator/S3VobGEsIEJqw7Zybg==|http://orcid.org/0000-0001-7374-4615
1000 Label
1000 Förderer
  1. Leibniz Institute for Farm Animals (FBN) |
  2. Leibniz Association |
1000 Fördernummer
  1. -
  2. -
1000 Förderprogramm
  1. Core budget
  2. Open Access fund
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Leibniz Institute for Farm Animals (FBN) |
    1000 Förderprogramm Core budget
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Leibniz Association |
    1000 Förderprogramm Open Access fund
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403641.rdf
1000 Erstellt am 2017-08-02T14:47:01.769+0200
1000 Erstellt von 122
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1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Mon Mar 01 08:28:52 CET 2021
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1000 Oai Id
  1. oai:frl.publisso.de:frl:6403641 |
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