Download
ncomms12347.pdf 2,77MB
WeightNameValue
1000 Titel
  • Sec16 alternative splicing dynamically controls COPII transport efficiency
1000 Autor/in
  1. Wilhelmi, Ilka |
  2. Kanski, Regina |
  3. Neumann, Alexander |
  4. Herdt, Olga |
  5. Hoff, Florian |
  6. Jacob, Ralf |
  7. Preußner, Marco |
  8. Heyd, Florian |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-08-05
1000 Erschienen in
1000 Quellenangabe
  • 7:12347
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1038/ncomms12347 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980449/ |
1000 Ergänzendes Material
  • http://www.nature.com/articles/ncomms12347#supplementary-information |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The transport of secretory proteins from the endoplasmic reticulum (ER) to the Golgi depends on COPII-coated vesicles. While the basic principles of the COPII machinery have been identified, it remains largely unknown how COPII transport is regulated to accommodate tissue- or activation-specific differences in cargo load and identity. Here we show that activation-induced alternative splicing of Sec16 controls adaptation of COPII transport to increased secretory cargo upon T-cell activation. Using splice-site blocking morpholinos and CRISPR/Cas9-mediated genome engineering, we show that the number of ER exit sites, COPII dynamics and transport efficiency depend on Sec16 alternative splicing. As the mechanistic basis, we suggest the C-terminal Sec16 domain to be a splicing-controlled protein interaction platform, with individual isoforms showing differential abilities to recruit COPII components. Our work connects the COPII pathway with alternative splicing, adding a new regulatory layer to protein secretion and its adaptation to changing cellular environments.
1000 Sacherschließung
lokal Coat complexes
lokal Protein translocation
lokal Alternative splicing
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/V2lsaGVsbWksIElsa2E=|https://frl.publisso.de/adhoc/creator/S2Fuc2tpLCBSZWdpbmE=|https://frl.publisso.de/adhoc/creator/TmV1bWFubiwgQWxleGFuZGVy|https://frl.publisso.de/adhoc/creator/SGVyZHQsIE9sZ2E=|https://frl.publisso.de/adhoc/creator/SG9mZiwgRmxvcmlhbg==|https://frl.publisso.de/adhoc/creator/SmFjb2IsIFJhbGY=|https://frl.publisso.de/adhoc/creator/UHJldcOfbmVyLCBNYXJjbw==|https://frl.publisso.de/adhoc/creator/SGV5ZCwgRmxvcmlhbg==
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
1000 Fördernummer
  1. HE 5398/3; SFB958/A21
1000 Förderprogramm
  1. Emmy-Noether-Fellowship
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm Emmy-Noether-Fellowship
    1000 Fördernummer HE 5398/3; SFB958/A21
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403655.rdf
1000 Erstellt am 2017-08-03T11:56:51.067+0200
1000 Erstellt von 122
1000 beschreibt frl:6403655
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet Thu Mar 04 08:35:13 CET 2021
1000 Objekt bearb. Thu Mar 04 08:35:12 CET 2021
1000 Vgl. frl:6403655
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403655 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source