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1000 Titel
  • Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A
1000 Autor/in
  1. Xu, Tianle |
  2. Shen, Xiangzhen |
  3. Seyfert, Hans-Martin |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-07-20
1000 Erschienen in
1000 Quellenangabe
  • 17:16
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • http://doi.org/10.1186/s12867-016-0069-5 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955114/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Stearoyl-CoA desaturase 1 (SCD1) desaturates long chain fatty acids and is therefore a key enzyme in fat catabolism. Its synthesis is downregulated in liver during illnesses caused by high levels of circulating lipopolysaccharide (LPS). SCD1 expression is known to be stimulated under adipogenic conditions through a variety of transcription factors, notably SREBP1 and C/EBPα and −β. However, mechanisms downregulating SCD1 expression during illness related reprograming of the metabolism were unknown. Escherichia coli elicited mastitis is an example of such a condition and was found to downregulates milk and milk fat synthesis. This is in part mediated through epigenetic mechanisms. We analyzed here mechanism controlling SCD1 expression in livers and udders from cows suffering from experimentally induced E. coli mastitis. RESULTS: We validated with RT-qPCR that SCD1 expression was reduced in these organs of the experimental cows. They also featured decreased levels of mRNAs encoding SREBP1a but increased levels for C/EBP α and −β. Chromatin accessibility PCR (CHART) revealed that downregulation of SCD1 expression in liver was not caused by tighter chromatin compaction of the SCD1 promoter. Reporter gene analyses showed in liver (HepG2) and mammary epithelial (MAC-T) model cells that overexpression of SREBP1a expectedly activated the promoter, while unexpectedly C/EBPα and −β strongly quenched the promoter activity. Abrogation of two from among of the three C/EBP DNA-binding motifs of the promoter revealed that C/EBPα acts in cis but C/EBPβ in trans. Overexpressing truncated C/EBPα or −β factors lacking their repressive domains confirmed in both model cells the direct action of C/EBPα, but not of C/EBPβ on the promoter. CONCLUSIONS: We found no evidence that epigenetic mechanism remodeling the chromatin compaction of the SCD1 promoter would contribute to downregulate SCD1 expression during infection. Instead, our data show for the first time that C/EBP factors may repress SCD1 expression in liver and udder rather than stimulating as it was previously shown in adipocytes. This cell type specific dual and opposite function of C/EBP factors for regulating SCD1 expression was previously unknown. Infection related activation of their expression combined with downregulated expression of SREBP1a explains reduced SCD1 expression in liver and udder during acute mastitis.
1000 Sacherschließung
lokal Liver
lokal Cattle
lokal SCD1
lokal C/EBP
lokal Fat metabolism
lokal Systemic reaction
lokal Udder
lokal Mastitis
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/WHUsIFRpYW5sZQ==|https://frl.publisso.de/adhoc/creator/U2hlbiwgWGlhbmd6aGVu|https://frl.publisso.de/adhoc/creator/U2V5ZmVydCwgSGFucy1NYXJ0aW4=
1000 Label
1000 Förderer
  1. National Natural Science Foundation of China |
  2. National Basic Research Program of China |
  3. Priority Academic Program Development of Jinagsu Higher Education Institutions (PAPD) |
1000 Fördernummer
  1. 31172371
  2. 2011CB100802
  3. -
1000 Förderprogramm
  1. -
  2. -
  3. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer National Natural Science Foundation of China |
    1000 Förderprogramm -
    1000 Fördernummer 31172371
  2. 1000 joinedFunding-child
    1000 Förderer National Basic Research Program of China |
    1000 Förderprogramm -
    1000 Fördernummer 2011CB100802
  3. 1000 joinedFunding-child
    1000 Förderer Priority Academic Program Development of Jinagsu Higher Education Institutions (PAPD) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
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1000 @id frl:6403728.rdf
1000 Erstellt am 2017-08-08T13:24:01.561+0200
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1000 Bearbeitet von 218
1000 Zuletzt bearbeitet 2021-08-05T13:13:35.381+0200
1000 Objekt bearb. Thu Aug 05 13:13:35 CEST 2021
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1000 Oai Id
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