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WeightNameValue
1000 Titel
  • Discovering miRNA Regulatory Networks in Holt–Oram Syndrome Using a Zebrafish Model
1000 Autor/in
  1. D’Aurizio, Romina |
  2. Russo, Francesco |
  3. Chiavacci, Elena |
  4. D’Onofrio, Mara |
  5. Arisi, Ivan |
  6. Rainaldi, Giuseppe |
  7. Pitto, Letizia |
  8. Pellegrini, Marco |
  9. Baumgart, Mario |
  10. Groth, Marco |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-07-14
1000 Erschienen in
1000 Quellenangabe
  • 4:60
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.3389/fbioe.2016.00060 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943955/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • MicroRNAs (miRNAs) are small non-coding RNAs that play an important role in the post-transcriptional regulation of gene expression. miRNAs are involved in the regulation of many biological processes such as differentiation, apoptosis, and cell proliferation. miRNAs are expressed in embryonic, postnatal, and adult hearts, and they have a key role in the regulation of gene expression during cardiovascular development and disease. Aberrant expression of miRNAs is associated with abnormal cardiac cell differentiation and dysfunction. Tbx5 is a member of the T-box gene family, which acts as transcription factor involved in the vertebrate heart development. Alteration of Tbx5 level affects the expression of hundreds of genes. Haploinsufficiency and gene duplication of Tbx5 are at the basis of the cardiac abnormalities associated with Holt–Oram syndrome (HOS). Recent data indicate that miRNAs might be an important part of the regulatory circuit through which Tbx5 controls heart development. Using high-throughput technologies, we characterized genome-widely the miRNA and mRNA expression profiles in WT- and Tbx5-depleted zebrafish embryos at two crucial developmental time points, 24 and 48 h post fertilization (hpf). We found that several miRNAs, which are potential effectors of Tbx5, are differentially expressed; some of them are already known to be involved in cardiac development and functions, such as miR-30, miR-34, miR-190, and miR-21. We performed an integrated analysis of miRNA expression data with gene expression profiles to refine computational target prediction approaches by means of the inversely correlation of miRNA–mRNA expressions, and we highlighted targets, which have roles in cardiac contractility, cardiomyocyte proliferation/apoptosis, and morphogenesis, crucial functions regulated by Tbx5. This approach allowed to discover complex regulatory circuits involving novel miRNAs and protein coding genes not considered before in the HOS such as miR-34a and miR-30 and their targets.
1000 Sacherschließung
lokal Holt–Oram
lokal NGS
lokal data integration
lokal microarray
lokal heart
lokal microRNA
lokal zebrafish
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/ROKAmUF1cml6aW8sIFJvbWluYQ==|https://frl.publisso.de/adhoc/creator/UnVzc28sIEZyYW5jZXNjbw==|https://frl.publisso.de/adhoc/creator/Q2hpYXZhY2NpLCBFbGVuYQ==|https://frl.publisso.de/adhoc/creator/ROKAmU9ub2ZyaW8sIE1hcmE=|https://frl.publisso.de/adhoc/creator/QXJpc2ksIEl2YW4=|https://frl.publisso.de/adhoc/creator/UmFpbmFsZGksIEdpdXNlcHBl|https://frl.publisso.de/adhoc/creator/UGl0dG8sIExldGl6aWE=|https://frl.publisso.de/adhoc/creator/UGVsbGVncmluaSwgTWFyY28=|http://orcid.org/0000-0001-8172-1595|http://orcid.org/0000-0002-9199-8990
1000 Label
1000 Förderer
  1. InterOmics |
  2. Italian Ministry for Education, University and Research (MIUR) |
  3. Italian National Research Council (CNR) |
  4. IIT-IFC Laboratory of Integrative Systems Medicine (LISM) |
1000 Fördernummer
  1. PB.P05, CUP B91J12000270001
  2. -
  3. -
  4. -
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer InterOmics |
    1000 Förderprogramm -
    1000 Fördernummer PB.P05, CUP B91J12000270001
  2. 1000 joinedFunding-child
    1000 Förderer Italian Ministry for Education, University and Research (MIUR) |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Italian National Research Council (CNR) |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer IIT-IFC Laboratory of Integrative Systems Medicine (LISM) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403740.rdf
1000 Erstellt am 2017-08-09T10:19:50.287+0200
1000 Erstellt von 122
1000 beschreibt frl:6403740
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet 2021-03-04T08:00:19.320+0100
1000 Objekt bearb. Thu Mar 04 08:00:18 CET 2021
1000 Vgl. frl:6403740
1000 Oai Id
  1. oai:frl.publisso.de:frl:6403740 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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