art_10.1186_s12864-016-2688-0.pdf 1,67MB
1000 Titel
  • A novel RNAseq–assisted method for MHC class I genotyping in a non-model species applied to a lethal vaccination-induced alloimmune disease
1000 Autor/in
  1. Demasius, Wiebke |
  2. Weikard, Rosemarie |
  3. Hadlich, Frieder |
  4. Buitkamp, Johannes |
  5. Kühn, Christa |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-05-17
1000 Erschienen in
1000 Quellenangabe
  • 17:365
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: MHC class I genotyping is essential for a wide range of biomedical, immunological and biodiversity applications. Whereas in human a comprehensive MHC class I allele catalogue is available, respective data in non-model species is scarce in spite of decades of research. RESULTS: Taking advantage of the new high-throughput RNA sequencing technology (RNAseq), we developed a novel RNAseq-assisted method (RAMHCIT) for MHC class I typing at nucleotide level. RAMHCIT is performed on white blood cells, which highly express MHC class I molecules enabling reliable discovery of new alleles and discrimination of closely related alleles due to the high coverage of alleles with reads. RAMHCIT is more comprehensive than previous methods, because no targeted PCR pre-amplification of MHC loci is necessary, which avoids preselection of alleles as usually encountered, when amplification with MHC class I primers is performed prior to sequencing. In addition to allele identification, RAMHCIT also enables quantification of MHC class I expression at allele level, which was remarkably consistent across individuals. CONCLUSIONS: Successful application of RAMHCIT is demonstrated on a data set from cattle with different phenotype regarding a lethal, vaccination-induced alloimmune disease (bovine neonatal pancytopenia), for which MHC class I alleles had been postulated as causal agents. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2688-0) contains supplementary material, which is available to authorized users.
1000 Sacherschließung
lokal MHC typing
lokal Cattle
lokal MHC class I
lokal Expression levels
lokal RNAseq
lokal Bovine neonatal pancytopenia (BNP)
1000 Fachgruppe
  1. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Förderer
  1. German Ministry of Food, Agriculture, and Consumer Protection (BMELV) |
1000 Fördernummer
  1. 2810HS27
1000 Förderprogramm
  1. Federal Office for Agriculture and Food (BLE)
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Ministry of Food, Agriculture, and Consumer Protection (BMELV) |
    1000 Förderprogramm Federal Office for Agriculture and Food (BLE)
    1000 Fördernummer 2810HS27
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403922.rdf
1000 Erstellt am 2017-08-16T13:02:57.127+0200
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1000 Vgl. frl:6403922
1000 Oai Id
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