PO_2016_11_2_Thormann.pdf 2,80MB
1000 Titel
  • Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae) of an Ecuadorian Mountain Forest Using DNA Barcoding
1000 Autor/in
  1. Thormann, Birthe |
  2. Ahrens, Dirk |
  3. Armijos, Diego Marín |
  4. Peters, Marcell K. |
  5. Wagner, Thomas |
  6. Wägele, Johann W. |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-02-05
1000 Erschienen in
1000 Quellenangabe
  • 11(2):e0148268
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient in southern Ecuador and explore their effect on species richness estimates. METHODOLOGY/PRINCIPAL FINDINGS: Based on a COI barcode data set of 674 leaf beetle specimens (Coleoptera: Chrysomelidae) of 266 morphospecies from three sample sites in the Podocarpus National Park, we employed statistical parsimony analysis, distance-based clustering, GMYC- and PTP-modelling to delimit species-like units and compared them to morphology-based (parataxonomic) species identifications. The four different approaches for DNA-based species delimitation revealed highly similar numbers of molecular operational taxonomic units (MOTUs) (n = 284–289). Estimated total species richness was considerably higher than the sampled amount, 414 for morphospecies (Chao2) and 469–481 for the different MOTU types. Assemblages at different elevational levels (1000 vs. 2000 m) had similar species numbers but a very distinct species composition for all delimitation methods. Most species were found only at one elevation while this turnover pattern was even more pronounced for DNA-based delimitation. CONCLUSION/SIGNIFICANCE: Given the high congruence of DNA-based delimitation results, probably due to the sampling structure, our study suggests that when applied to species communities on a regionally limited level with high amount of rare species (i.e. ~50% singletons), the choice of species delimitation method can be of minor relevance for assessing species numbers and turnover in tropical insect communities. Therefore, DNA-based species delimitation is confirmed as a valuable tool for evaluating biodiversity of hyperdiverse insect communities, especially when exact taxonomic identifications are missing.
1000 Sacherschließung
lokal Beetles
lokal Taxonomy
lokal Biodiversity
lokal Haplotypes
lokal Species delimitation
lokal Species diversity
lokal Endangered species
lokal Insects
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. German Science Foundation (DFG) |
1000 Fördernummer
  1. WA 530/46-1
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Science Foundation (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer WA 530/46-1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403988.rdf
1000 Erstellt am 2017-08-18T11:22:30.819+0200
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1000 Zuletzt bearbeitet Thu Dec 03 12:20:41 CET 2020
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