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1000 Titel
  • Comparison of the pathogen species-specific immune response in udder derived cell types and their models
1000 Autor/in
  1. Günther, Juliane |
  2. Koy, Mirja |
  3. Berthold, Anne |
  4. Schuberth, Hans-Joachim |
  5. Seyfert, Hans-Martin |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-02-01
1000 Erschienen in
1000 Quellenangabe
  • 47:22
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1186/s13567-016-0307-3 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736154/ |
1000 Ergänzendes Material
  • https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-016-0307-3#Declarations |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The outcome of an udder infection (mastitis) largely depends on the species of the invading pathogen. Gram-negative pathogens, such as Escherichia coli often elicit acute clinical mastitis while Gram-positive pathogens, such as Staphylococcus aureus tend to cause milder subclinical inflammations. It is unclear which type of the immune competent cells residing in the udder governs the pathogen species-specific physiology of mastitis and which established cell lines might provide suitable models. We therefore profiled the pathogen species-specific immune response of different cell types derived from udder and blood. Primary cultures of bovine mammary epithelial cells (pbMEC), mammary derived fibroblasts (pbMFC), and bovine monocyte-derived macrophages (boMdM) were challenged with heat-killed E. coli, S. aureus and S. uberis mastitis pathogens and their immune response was scaled against the response of established models for MEC (bovine MAC-T) and macrophages (murine RAW 264.7). Only E. coli provoked a full scale immune reaction in pbMEC, fibroblasts and MAC-T cells, as indicated by induced cytokine and chemokine expression and NF-?B activation. Weak reactions were induced by S. aureus and none by S. uberis challenges. In contrast, both models for macrophages (boMdM and RAW 264.7) reacted strongly against all the three pathogens accompanied by strong activation of NF-?B factors. Hence, the established cell models MAC-T and RAW 264.7 properly reflected key aspects of the pathogen species-specific immune response of the respective parental cell type. Our data imply that the pathogen species-specific physiology of mastitis likely relates to the respective response of MEC rather to that of professional immune cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-016-0307-3) contains supplementary material, which is available to authorized users.
1000 Fachgruppe
  1. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R8O8bnRoZXIsIEp1bGlhbmU=|https://frl.publisso.de/adhoc/creator/S295LCBNaXJqYQ==|https://frl.publisso.de/adhoc/creator/QmVydGhvbGQsIEFubmU=|https://frl.publisso.de/adhoc/creator/U2NodWJlcnRoLCBIYW5zLUpvYWNoaW0=|https://frl.publisso.de/adhoc/creator/U2V5ZmVydCwgSGFucy1NYXJ0aW4=
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
1000 Fördernummer
  1. GU 1487/1-1
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer GU 1487/1-1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6403996.rdf
1000 Erstellt am 2017-08-18T12:34:50.102+0200
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1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 07 12:10:09 CEST 2018
1000 Objekt bearb. Mon May 07 12:10:09 CEST 2018
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1000 Oai Id
  1. oai:frl.publisso.de:frl:6403996 |
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