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Multiplex sequencing of bacterial artificial chromosomes for assembling complex plant genomes.pdf 673,51KB
WeightNameValue
1000 Titel
  • Multiplex sequencing of bacterial artificial chromosomes for assembling complex plant genomes
1000 Autor/in
  1. Beier, Sebastian |
  2. Himmelbach, Axel |
  3. Schmutzer, Thomas |
  4. Felder, Marius |
  5. Taudien, Stefan |
  6. Mayer, Klaus F. X. |
  7. Platzer, Matthias |
  8. Stein, Nils |
  9. Scholz, Uwe |
  10. Mascher, Martin |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-01-23
1000 Erschienen in
1000 Quellenangabe
  • 14(7): 1511-1522
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1111/pbi.12511 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066668/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Hierarchical shotgun sequencing remains the method of choice for assembling high-quality reference sequences of complex plant genomes. The efficient exploitation of current high-throughput technologies and powerful computational facilities for large-insert clone sequencing necessitates the sequencing and assembly of a large number of clones in parallel. We developed a multiplexed pipeline for shotgun sequencing and assembling individual bacterial artificial chromosomes (BACs) using the Illumina sequencing platform. We illustrate our approach by sequencing 668 barley BACs (Hordeum vulgare L.) in a single Illumina HiSeq 2000 lane. Using a newly designed parallelized computational pipeline, we obtained sequence assemblies of individual BACs that consist, on average, of eight sequence scaffolds and represent >98% of the genomic inserts. Our BAC assemblies are clearly superior to a whole-genome shotgun assembly regarding contiguity, completeness and the representation of the gene space. Our methods may be employed to rapidly obtain high-quality assemblies of a large number of clones to assemble map-based reference sequences of plant and animal species with complex genomes by sequencing along a minimum tiling path.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. http://orcid.org/0000-0002-2177-8781|http://orcid.org/0000-0001-7338-0946|http://orcid.org/0000-0003-1073-6719|https://frl.publisso.de/adhoc/creator/RmVsZGVyLCBNYXJpdXM=|https://frl.publisso.de/adhoc/creator/VGF1ZGllbiwgU3RlZmFu|https://frl.publisso.de/adhoc/creator/TWF5ZXIsIEtsYXVzIEYuIFgu|http://orcid.org/0000-0003-0596-8582|http://orcid.org/0000-0003-3011-8731|http://orcid.org/0000-0001-6113-3518|http://orcid.org/0000-0001-6373-6013
1000 Förderer
  1. German Ministry of Education and Research |
  2. German Leibniz Association |
1000 Fördernummer
  1. FKZ0314000; FKZ0315954
  2. -
1000 Förderprogramm
  1. BARLEX; TRITEX
  2. Pakt für Forschung und Innovation
1000 Dateien
  1. Multiplex sequencing of bacterial artificial chromosomes for assembling complex plant genomes
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Ministry of Education and Research |
    1000 Förderprogramm BARLEX; TRITEX
    1000 Fördernummer FKZ0314000; FKZ0315954
  2. 1000 joinedFunding-child
    1000 Förderer German Leibniz Association |
    1000 Förderprogramm Pakt für Forschung und Innovation
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404131.rdf
1000 Erstellt am 2017-08-25T10:31:44.871+0200
1000 Erstellt von 122
1000 beschreibt frl:6404131
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Tue Mar 13 09:08:44 CET 2018
1000 Objekt bearb. Tue Mar 13 09:08:44 CET 2018
1000 Vgl. frl:6404131
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404131 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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