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1000 Titel
  • Physiological Concentration of Exogenous Lactate Reduces Antimycin A Triggered Oxidative Stress in Intestinal Epithelial Cell Line IPEC-1 and IPEC-J2 In Vitro
1000 Autor/in
  1. Kahlert, Stefan |
  2. Junnikkala, Sami |
  3. Renner, Lydia |
  4. Hynönen, Ulla |
  5. Hartig, Roland |
  6. Nossol, Constanze |
  7. Barta-Böszörményi, Anikó |
  8. Dänicke, Sven |
  9. Souffrant, Wolfgang-Bernhard |
  10. Palva, Airi |
  11. Rothkötter, Hermann-Josef |
  12. Kluess, Jeannette |
1000 Erscheinungsjahr 2016
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-04-07
1000 Erschienen in
1000 Quellenangabe
  • 11(4): e0153135
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0153135 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824430/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Weaning triggers an adaptation of the gut function including luminal lactate generation by lactobacilli, depending on gastrointestinal site. We hypothesized that both lactobacilli and lactate influence porcine intestinal epithelial cells. In vivo experiments showed that concentration of lactate was significantly higher in gastric, duodenal and jejunal chyme of suckling piglets compared to their weaned counterparts. In an in vitro study we investigated the impact of physiological lactate concentration as derived from the in vivo study on the porcine intestinal epithelial cells IPEC-1 and IPEC-J2. We detected direct adherence of lactobacilli on the apical epithelial surface and a modulated F-actin structure. Application of lactobacilli culture supernatant alone or lactate (25 mM) at low pH (pH 4) changed the F-actin structure in a similar manner. Treatment of IPEC cultures with lactate at near neutral pH resulted in a significantly reduced superoxide-generation in Antimycin A-challenged cells. This protective effect was nearly completely reversed by inhibition of cellular lactate uptake via monocarboxylate transporter. Lactate treatment enhanced NADH autofluorescence ratio (Fcytosol/Fnucleus) in non-challenged cells, indicating an increased availability of reduced nucleotides, but did not change the overall ATP content of the cells. Lactobacilli-derived physiological lactate concentration in intestine is relevant for alleviation of redox stress in intestinal epithelial cells.
1000 Sacherschließung
lokal Mitochondria
lokal Jejunum
lokal Swine
lokal Superoxides
lokal Gastrointestinal tract
lokal Colon
lokal Glucose
lokal Lactobacillus
1000 Fachgruppe
  1. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S2FobGVydCwgU3RlZmFu|https://frl.publisso.de/adhoc/creator/SnVubmlra2FsYSwgU2FtaQ==|https://frl.publisso.de/adhoc/creator/UmVubmVyLCBMeWRpYQ==|https://frl.publisso.de/adhoc/creator/SHluw7ZuZW4sIFVsbGE=|https://frl.publisso.de/adhoc/creator/SGFydGlnLCBSb2xhbmQ=|https://frl.publisso.de/adhoc/creator/Tm9zc29sLCBDb25zdGFuemU=|https://frl.publisso.de/adhoc/creator/QmFydGEtQsO2c3rDtnJtw6lueWksIEFuaWvDsw==|https://frl.publisso.de/adhoc/creator/RMOkbmlja2UsIFN2ZW4=|https://frl.publisso.de/adhoc/creator/U291ZmZyYW50LCBXb2xmZ2FuZy1CZXJuaGFyZA==|https://frl.publisso.de/adhoc/creator/UGFsdmEsIEFpcmk=|https://frl.publisso.de/adhoc/creator/Um90aGvDtnR0ZXIsIEhlcm1hbm4tSm9zZWY=|https://frl.publisso.de/adhoc/creator/S2x1ZXNzLCBKZWFubmV0dGU=
1000 Förderer
  1. European Union |
1000 Fördernummer
  1. QLK5-CT-2000-00522; 227549
1000 Förderprogramm
  1. 5th frame-work program "HealthyPigut"; 7th frame-work program INTERPLAY
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm 5th frame-work program "HealthyPigut"; 7th frame-work program INTERPLAY
    1000 Fördernummer QLK5-CT-2000-00522; 227549
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404214.rdf
1000 Erstellt am 2017-08-28T13:32:27.418+0200
1000 Erstellt von 218
1000 beschreibt frl:6404214
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Jun 13 11:13:53 CEST 2018
1000 Objekt bearb. Wed Jun 13 11:13:53 CEST 2018
1000 Vgl. frl:6404214
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404214 |
1000 Sichtbarkeit Metadaten public
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1000 Gegenstand von

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