Analysis of factors contributing to variation in the C57BL/6J fecal microbiota across German animal facilities

  1. Rausch, Philipp
  2. Basic, Marijana
  3. Batra, Arvind
  4. Bischoff, Stephan C.
  5. Blaut, Michael
  6. Clavel, Thomas
  7. Gläsner, Joachim
  8. Gopalakrishnan, Shreya
  9. Grassl, Guntram A.
  10. Günther, Claudia
  11. Haller, Dirk
  12. Hirose, Misa
  13. Ibrahim, Saleh
  14. Loh, Gunnar
  15. Mattner, Jochen
  16. Nagel, Stefan
  17. Pabst, Oliver
  18. Schmidt, Franziska
  19. Siegmund, Britta
  20. Strowig, Till
  21. Volynets, Valentina
  22. Wirtz, Stefan
  23. Zeissig, Sebastian
  24. Zeissig, Yvonne
  25. Bleich, André
  26. Baines, John F.

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WeightNameValue
1000 Titel
  • Analysis of factors contributing to variation in the C57BL/6J fecal microbiota across German animal facilities
1000 Autor/in
  1. Rausch, Philipp |
  2. Basic, Marijana |
  3. Batra, Arvind |
  4. Bischoff, Stephan C. |
  5. Blaut, Michael |
  6. Clavel, Thomas |
  7. Gläsner, Joachim |
  8. Gopalakrishnan, Shreya |
  9. Grassl, Guntram A. |
  10. Günther, Claudia |
  11. Haller, Dirk |
  12. Hirose, Misa |
  13. Ibrahim, Saleh |
  14. Loh, Gunnar |
  15. Mattner, Jochen |
  16. Nagel, Stefan |
  17. Pabst, Oliver |
  18. Schmidt, Franziska |
  19. Siegmund, Britta |
  20. Strowig, Till |
  21. Volynets, Valentina |
  22. Wirtz, Stefan |
  23. Zeissig, Sebastian |
  24. Zeissig, Yvonne |
  25. Bleich, André |
  26. Baines, John F. |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-03-15
1000 Erschienen in
1000 Quellenangabe
  • 306(5): 343-355
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1016/j.ijmm.2016.03.004 |
1000 Ergänzendes Material
  • http://www.sciencedirect.com/science/article/pii/S1438422116300194?via%3Dihub#sec0070 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The intestinal microbiota is involved in many physiological processes and it is increasingly recognized that differences in community composition can influence the outcome of a variety of murine models used in biomedical research. In an effort to describe and account for the variation in intestinal microbiota composition across the animal facilities of participating members of the DFG Priority Program 1656 “Intestinal Microbiota”, we performed a survey of C57BL/6J mice from 21 different mouse rooms/facilities located at 13 different institutions across Germany. Fresh feces was sampled from five mice per room/facility using standardized procedures, followed by extraction and 16S rRNA gene profiling (V1–V2 region, Illumina MiSeq) at both the DNA and RNA (reverse transcribed to cDNA) level. In order to determine the variables contributing to bacterial community differences, we collected detailed questionnaires of animal husbandry practices and incorporated this information into our analyses. We identified considerable variation in a number of descriptive aspects including the proportions of major phyla, alpha- and beta diversity, all of which displayed significant associations to specific aspects of husbandry. Salient findings include a reduction in alpha diversity with the use of irradiated chow, an increase in inter-individual variability (beta diversity) with respect to barrier access and open cages and an increase in bacterial community divergence with time since importing from a vendor. We further observe a high degree of facility-level individuality, which is likely due to each facility harboring its own unique combination of multiple varying attributes of animal husbandry. While it is important to account and control for such differences between facilities, the documentation of such diversity may also serve as a valuable future resource for investigating the origins of microbial-driven host phenotypes.
1000 Sacherschließung
lokal 16S rRNA gene
lokal Gut microbiota
lokal Mouse husbandry
lokal C57BL/6J
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
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1000 Label
1000 Förderer
  1. German Research Foundation (DFG) |
  2. Max Planck Society |
1000 Fördernummer
  1. BA 2863/5-1
  2. -
1000 Förderprogramm
  1. Priority Program SPP 1656 “Intestinal Microbiota- A Microbial Ecosystem at the Edge between Immune Homeostasis and Inflammation”; Excellence Cluster 306 “Inflammation at Interfaces”
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation (DFG) |
    1000 Förderprogramm Priority Program SPP 1656 “Intestinal Microbiota- A Microbial Ecosystem at the Edge between Immune Homeostasis and Inflammation”; Excellence Cluster 306 “Inflammation at Interfaces”
    1000 Fördernummer BA 2863/5-1
  2. 1000 joinedFunding-child
    1000 Förderer Max Planck Society |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404337.rdf
1000 Erstellt am 2017-09-08T14:19:55.356+0200
1000 Erstellt von 218
1000 beschreibt frl:6404337
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet 2021-03-04T12:07:54.769+0100
1000 Objekt bearb. Thu Mar 04 12:07:54 CET 2021
1000 Vgl. frl:6404337
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404337 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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