WeightNameValue
1000 Titel
  • Ultrafast folding of WW domains without structured aromatic clusters in the denatured state
1000 Autor/in
  1. Ferguson, Neil |
  2. Johnson, Christopher M. |
  3. Macias, Maria |
  4. Oschkinat, Hartmut |
  5. Fersht, Alan |
1000 Erscheinungsjahr 2001
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2001-10-30
1000 Erschienen in
1000 Quellenangabe
  • 98(23): 13002-13007
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC60814/ |
  • https://dx.doi.org/10.1073/pnas.221467198 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Ultrafast-folding proteins are important for combining experiment and simulation to give complete descriptions of folding pathways. The WW domain family comprises small proteins with a three-stranded antiparallel β-sheet topology. Previous studies on the 57-residue YAP 65 WW domain indicate the presence of residual structure in the chemically denatured state. Here we analyze three minimal core WW domains of 38–44 residues. There was little spectroscopic or thermodynamic evidence for residual structure in either their chemically or thermally denatured states. Folding and unfolding kinetics, studied by using rapid temperature-jump and continuous-flow techniques, show that each domain folds and unfolds very rapidly in a two-state transition through a highly compact transition state. Folding half-times were as short as 17 μs at 25°C, within an order of magnitude of the predicted maximal rate of loop formation. The small size and topological simplicity of these domains, in conjunction with their very rapid two-state folding, may allow us to reduce the difference in time scale between experiment and theoretical simulation.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/RmVyZ3Vzb24sIE5laWw=|https://frl.publisso.de/adhoc/creator/Sm9obnNvbiwgQ2hyaXN0b3BoZXIgTS4=|https://frl.publisso.de/adhoc/creator/TWFjaWFzLCBNYXJpYQ==|https://frl.publisso.de/adhoc/creator/T3NjaGtpbmF0LCBIYXJ0bXV0|https://frl.publisso.de/adhoc/creator/RmVyc2h0LCBBbGFu
1000 Förderer
  1. Medical Research Council, Cambridge, U.K |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Medical Research Council, Cambridge, U.K |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404468.rdf
1000 Erstellt am 2017-09-15T13:09:59.564+0200
1000 Erstellt von 122
1000 beschreibt frl:6404468
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Aug 01 12:42:58 CEST 2018
1000 Objekt bearb. Wed Aug 01 12:42:58 CEST 2018
1000 Vgl. frl:6404468
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404468 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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