WeightNameValue
1000 Titel
  • Using flexible loop mimetics to extend Φ-value analysis to secondary structure interactions
1000 Autor/in
  1. Ferguson, Neil |
  2. Pires, José Ricardo |
  3. Toepert, Florian |
  4. Johnson, Christopher M. |
  5. Pan, Yong Ping |
  6. Volkmer-Engert, Rudolf |
  7. Schneider-Mergener, Jens |
  8. Daggett, Valerie |
  9. Oschkinat, Hartmut |
  10. Fersht, Alan |
1000 Erscheinungsjahr 2001
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2001-10-30
1000 Erschienen in
1000 Quellenangabe
  • 98(23): 13008-13013
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC60815/ |
  • https://dx.doi.org/10.1073/pnas.221467398 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Chemical synthesis allows the incorporation of nonnatural amino acids into proteins that may provide previously untried probes of their folding pathway and thermodynamic stability. We have used a flexible thioether linker as a loop mimetic in the human yes kinase-associated protein (YAP 65) WW domain, a three-stranded, 44-residue, β-sheet protein. This linkage avoids problems of incorporating sequences that constrain loops to the extent that they significantly change the nature of the denatured state with concomitant effects on the folding kinetics. An NMR solution structure shows that the thioether linker had little effect on the global fold of the domain, although the loop is apparently more dynamic. The thioether variants are destabilized by up to 1.4 kcal/mol (1 cal = 4.18 J). Preliminary Φ-value analysis showed that the first loop is highly structured in the folding transition state, and the second loop is essentially unstructured. These data are consistent with results from simulated unfolding and detailed protein-engineering studies of structurally homologous WW domains. Previously, Φ-value analysis was limited to studying side-chain interactions. The linkers used here extend the protein engineering method directly to secondary-structure interactions.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/RmVyZ3Vzb24sIE5laWw=|https://frl.publisso.de/adhoc/creator/UGlyZXMsIEpvc8OpIFJpY2FyZG8=|https://frl.publisso.de/adhoc/creator/VG9lcGVydCwgRmxvcmlhbg==|https://frl.publisso.de/adhoc/creator/Sm9obnNvbiwgQ2hyaXN0b3BoZXIgTS4=|https://frl.publisso.de/adhoc/creator/UGFuLCBZb25nIFBpbmc=|https://frl.publisso.de/adhoc/creator/Vm9sa21lci1FbmdlcnQsIFJ1ZG9sZg==|https://frl.publisso.de/adhoc/creator/U2NobmVpZGVyLU1lcmdlbmVyLCBKZW5z|https://frl.publisso.de/adhoc/creator/RGFnZ2V0dCwgVmFsZXJpZQ==|https://frl.publisso.de/adhoc/creator/T3NjaGtpbmF0LCBIYXJ0bXV0|https://frl.publisso.de/adhoc/creator/RmVyc2h0LCBBbGFu
1000 Label
1000 Förderer
  1. Medical Research Council, United Kingdom |
  2. Deutsche Forschungsgemeinschaft |
  3. Deutsche Akademische Austauschdienst |
  4. National Institutes of Health |
1000 Fördernummer
  1. -
  2. SCHN 317/6–7
  3. -
  4. GM 50789
1000 Förderprogramm
  1. -
  2. -
  3. fellowship
  4. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Medical Research Council, United Kingdom |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SCHN 317/6–7
  3. 1000 joinedFunding-child
    1000 Förderer Deutsche Akademische Austauschdienst |
    1000 Förderprogramm fellowship
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer National Institutes of Health |
    1000 Förderprogramm -
    1000 Fördernummer GM 50789
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404469.rdf
1000 Erstellt am 2017-09-15T13:20:10.188+0200
1000 Erstellt von 122
1000 beschreibt frl:6404469
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Thu Jan 30 23:18:18 CET 2020
1000 Objekt bearb. Fri Sep 15 13:20:16 CEST 2017
1000 Vgl. frl:6404469
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404469 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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