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WeightNameValue
1000 Titel
  • The Bacterial Translocon SecYEG Opens upon Ribosome Binding
1000 Autor/in
  1. Knyazev, Denis G. |
  2. Lents, Alexander |
  3. Krause, Eberhard |
  4. Ollinger, Nicole |
  5. Siligan, Christine |
  6. Papinski, Daniel |
  7. Winter, Lukas |
  8. Horner, Andreas |
  9. Pohl, Peter |
1000 Erscheinungsjahr 2013
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2013-05-03
1000 Erschienen in
1000 Quellenangabe
  • 288(25): 17941–17946
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2013
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1074/jbc.M113.477893 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689939/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • In co-translational translocation, the ribosome funnel and the channel of the protein translocation complex SecYEG are aligned. For the nascent chain to enter the channel immediately after synthesis, a yet unidentified signal triggers displacement of the SecYEG sealing plug from the pore. Here, we show that ribosome binding to the resting SecYEG channel triggers this conformational transition. The purified and reconstituted SecYEG channel opens to form a large ion-conducting channel, which has the conductivity of the plug deletion mutant. The number of ion-conducting channels inserted into the planar bilayer per fusion event roughly equals the number of SecYEG channels counted by fluorescence correlation spectroscopy in a single proteoliposome. Thus, the open probability of the channel must be close to unity. To prevent the otherwise lethal proton leak, a closed post-translational conformation of the SecYEG complex bound to a ribosome must exist.
1000 Sacherschließung
lokal Protein Translocation
lokal Channel Gating
lokal Membrane Biophysics
lokal Membrane Bilayer
lokal Permeability
lokal Membrane Reconstitution
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S255YXpldiwgRGVuaXMgRy4=|https://frl.publisso.de/adhoc/creator/TGVudHMsIEFsZXhhbmRlcg==|https://frl.publisso.de/adhoc/creator/S3JhdXNlLCBFYmVyaGFyZA==|https://frl.publisso.de/adhoc/creator/T2xsaW5nZXIsIE5pY29sZQ==|https://frl.publisso.de/adhoc/creator/U2lsaWdhbiwgQ2hyaXN0aW5l|https://frl.publisso.de/adhoc/creator/UGFwaW5za2ksIERhbmllbA==|https://frl.publisso.de/adhoc/creator/V2ludGVyLCBMdWthcw==|https://frl.publisso.de/adhoc/creator/SG9ybmVyLCBBbmRyZWFz|http://orcid.org/0000-0002-1792-2314
1000 Förderer
  1. Austrian federal government |
1000 Fördernummer
  1. P20872
1000 Förderprogramm
  1. Austrian Science Fund (FWF)
1000 Dateien
  1. The Bacterial Translocon SecYEG Opens upon Ribosome Binding
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Austrian federal government |
    1000 Förderprogramm Austrian Science Fund (FWF)
    1000 Fördernummer P20872
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404588.rdf
1000 Erstellt am 2017-09-22T12:05:52.643+0200
1000 Erstellt von 218
1000 beschreibt frl:6404588
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Aug 01 11:45:05 CEST 2018
1000 Objekt bearb. Wed Aug 01 11:45:05 CEST 2018
1000 Vgl. frl:6404588
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404588 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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