pone.0058306.pdf 416,49KB
1000 Titel
  • Transcriptomic Response of Porcine PBMCs to Vaccination with Tetanus Toxoid as a Model Antigen
1000 Autor/in
  1. Adler, Marcel |
  2. Murani, Eduard |
  3. Brunner, Ronald |
  4. Ponsuksili, Siriluck |
  5. Wimmers, Klaus |
1000 Erscheinungsjahr 2013
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2013-03-25
1000 Erschienen in
1000 Quellenangabe
  • 8(3): e58306
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2013
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The aim of the present study was to characterize in vivo genome-wide transcriptional responses to immune stimulation in order to get insight into the resulting changes of allocation of resources. Vaccination with tetanus toxoid was used as a model for a mixed Th1 and Th2 immune response in pig. Expression profiles of PBMCs (peripheral blood mononuclear cells) before and at 12 time points over a period of four weeks after initial and booster vaccination at day 14 were studied by use of Affymetrix GeneChip microarrays and Ingenuity Pathway Analysis (IPA). The transcriptome data in total comprised more than 5000 genes with different transcript abundances (DE-genes). Within the single time stages the numbers of DE-genes were between several hundred and more than 1000. Ingenuity Pathway Analysis mainly revealed canonical pathways of cellular immune response and cytokine signaling as well as a broad range of processes in cellular and organismal growth, proliferation and development, cell signaling, biosynthesis and metabolism. Significant changes in the expression profiles of PBMCs already occurred very early after immune stimulation. At two hours after the first vaccination 679 DE-genes corresponding to 110 canonical pathways of cytokine signaling, cellular immune response and other multiple cellular functions were found. Immune competence and global disease resistance are heritable but difficult to measure and to address by breeding. Besides QTL mapping of immune traits gene expression profiling facilitates the detection of functional gene networks and thus functional candidate genes.
1000 Sacherschließung
lokal Developmental signaling
lokal Immune response
lokal VEGF signaling
lokal Vaccination and immunization
lokal Microarrays
lokal T cells
lokal Cell signaling
lokal Immune receptor signaling
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. German Federal Ministry of Education and Research (BMBF) |
1000 Fördernummer
  1. FKZ 0315130 A
1000 Förderprogramm
  1. project GeneDialog: FUGATOplus
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Federal Ministry of Education and Research (BMBF) |
    1000 Förderprogramm project GeneDialog: FUGATOplus
    1000 Fördernummer FKZ 0315130 A
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404598.rdf
1000 Erstellt am 2017-09-22T12:39:40.614+0200
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