1471-2164-14-36.pdf 997,77KB
1000 Titel
  • Escherichia coli- and Staphylococcus aureus-induced mastitis differentially modulate transcriptional responses in neighbouring uninfected bovine mammary gland quarters
1000 Autor/in
  1. Jensen, Kirsty |
  2. Günther, Juliane |
  3. Talbot, Richard |
  4. Petzl, Wolfram |
  5. Zerbe, Holm |
  6. Schuberth, Hans-Joachim |
  7. Seyfert, Hans-Martin |
  8. Glass, Elizabeth J. |
1000 Erscheinungsjahr 2013
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2013-01-16
1000 Erschienen in
1000 Quellenangabe
  • 14: 36
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2013
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: The most important disease of dairy cattle is mastitis, caused by the infection of the mammary gland by various micro-organisms. Although the transcriptional response of bovine mammary gland cells to in vitro infection has been studied, the interplay and consequences of these responses in the in vivo environment of the mammary gland are less clear. Previously mammary gland quarters were considered to be unaffected by events occurring in neighbouring quarters. More recently infection of individual quarters with mastitis causing pathogens, especially Escherichia coli, has been shown to influence the physiology of neighbouring uninfected quarters. Therefore, the transcriptional responses of uninfected mammary gland quarters adjacent to quarters infected with two major mastitis causing pathogens, E. coli and Staphylococcus aureus, were compared. RESULTS: The bacteriologically sterile, within-animal control quarters exhibited a transcriptional response to the infection of neighbouring quarters. The greatest response was associated with E. coli infection, while a weaker, yet significant, response occurred during S. aureus infection. The transcriptional responses of these uninfected quarters included the enhanced expression of many genes previously associated with mammary gland infections. Comparison of the transcriptional response of uninfected quarters to S. aureus and E. coli infection identified 187 differentially expressed genes, which were particularly associated with cellular responses, e.g. response to stress. The most affected network identified by Ingenuity Pathway analysis has the immunosuppressor transforming growth factor beta 1 (TGFB1) at its hub and largely consists of genes more highly expressed in control quarters from S. aureus infected cows. CONCLUSIONS: Uninfected mammary gland quarters reacted to the infection of neighbouring quarters and the responses were dependent on pathogen type. Therefore, bovine udder quarters exhibit interdependence and should not be considered as separate functional entities. This suggests that mastitis pathogens not only interact directly with host mammary cells, but also influence discrete sites some distance away, which will affect their response to the subsequent spread of the infection. Understanding the underlying mechanisms may provide further clues for ways to control mammary gland infections. These results also have implications for the design of experimental studies investigating immune regulatory mechanisms in the bovine mammary gland.
1000 Sacherschließung
lokal Mastitis
lokal Bovine
lokal Escherichia coli
lokal Microarray
lokal Staphylococcus aureus
1000 Fachgruppe
  1. Biologie |
  2. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. European Animal Disease Genomics Network of Excellence for Animal Health and Food Safety (EADGENE) |
  2. Biotechnology and Biological Sciences Research Council |
  3. Deutsche Forschungsgemeinschaft (DFG) |
1000 Fördernummer
  1. FOOD-CT-2004-506416
  2. -
  3. Se 326/14-3
1000 Förderprogramm
  1. -
  2. Institute Strategic Programme Grant
  3. Researcher Group FOR585
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Animal Disease Genomics Network of Excellence for Animal Health and Food Safety (EADGENE) |
    1000 Förderprogramm -
    1000 Fördernummer FOOD-CT-2004-506416
  2. 1000 joinedFunding-child
    1000 Förderer Biotechnology and Biological Sciences Research Council |
    1000 Förderprogramm Institute Strategic Programme Grant
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm Researcher Group FOR585
    1000 Fördernummer Se 326/14-3
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404630.rdf
1000 Erstellt am 2017-09-25T10:11:55.421+0200
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1000 Zuletzt bearbeitet Thu Jan 30 23:18:48 CET 2020
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