WeightNameValue
1000 Titel
  • The Proteoglycan Syndecan 4 Regulates Transient Receptor Potential Canonical 6 Channels via RhoA/Rho-associated Protein Kinase Signaling
1000 Autor/in
  1. Liu, Ying |
  2. Echtermeyer, Frank |
  3. Thilo, Florian |
  4. Theilmeier, Gregor |
  5. Schmidt, Antje |
  6. Schülein, Ralf |
  7. Jensen, Boye L. |
  8. Loddenkemper, Christoph |
  9. Jankowski, Vera |
  10. Marcussen, Niels |
  11. Gollasch, Maik |
  12. Arendshorst, William J. |
  13. Tepel, Martin |
1000 Erscheinungsjahr 2011
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2011-12-08
1000 Erschienen in
1000 Quellenangabe
  • 32(2): 378-385
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881556/ |
  • https://doi.org/10.1161/ATVBAHA.111.241018 |
1000 Ergänzendes Material
  • http://atvb.ahajournals.org/content/32/2/378/tab-supplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • OBJECTIVE:Syndecan 4 (Sdc4) modulates signal transduction and regulates activity of protein channels. Sdc4 is essential for the regulation of cellular permeability. We hypothesized that Sdc4 may regulate transient receptor potential canonical 6 (TRPC6) channels, a determinant of glomerular permeability, in a RhoA/Rho-associated protein kinase-dependent manner. METHODS AND RESULTS:Sdc4 knockout (Sdc4−/−) mice showed increased glomerular filtration rate and ameliorated albuminuria under baseline conditions and after bovine serum albumin overload (each P<0.05). Using reverse transcription–polymerase chain reaction and immunoblotting, Sdc4−/− mice showed reduced TRPC6 mRNA by 79% and TRPC6 protein by 82% (each P<0.05). Sdc4−/− mice showed an increased RhoA activity by 87% and increased phosphorylation of ezrin in glomeruli by 48% (each P<0.05). Sdc4 knockdown in cultured podocytes reduced TRPC6 gene expression and reduced the association of TRPC6 with plasma membrane and TRPC6-mediated calcium influx and currents. Sdc4 knockdown inactivated negative regulatory protein Rho GTPase activating protein by 33%, accompanied by a 41% increase in RhoA activity and increased phosphorylation of ezrin (P<0.05). Conversely, overexpression of Sdc4 reduced RhoA activity and increased TRPC6 protein and TRPC6-mediated calcium influx and currents. CONCLUSION: Our results establish a previously unknown function of Sdc4 for regulation of TRPC6 channels and support the role of Sdc4 for the regulation of glomerular permeability.
1000 Sacherschließung
lokal receptors
lokal signal transduction
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TGl1LCBZaW5n|https://frl.publisso.de/adhoc/creator/RWNodGVybWV5ZXIsIEZyYW5r|https://frl.publisso.de/adhoc/creator/VGhpbG8sIEZsb3JpYW4=|https://frl.publisso.de/adhoc/creator/VGhlaWxtZWllciwgR3JlZ29y|https://frl.publisso.de/adhoc/creator/U2NobWlkdCwgQW50amU=|https://frl.publisso.de/adhoc/creator/U2Now7xsZWluLCBSYWxm|https://frl.publisso.de/adhoc/creator/SmVuc2VuLCBCb3llIEwu|https://frl.publisso.de/adhoc/creator/TG9kZGVua2VtcGVyLCBDaHJpc3RvcGg=|https://frl.publisso.de/adhoc/creator/SmFua293c2tpLCBWZXJh|https://frl.publisso.de/adhoc/creator/TWFyY3Vzc2VuLCBOaWVscw==|https://frl.publisso.de/adhoc/creator/R29sbGFzY2gsIE1haWs=|https://frl.publisso.de/adhoc/creator/QXJlbmRzaG9yc3QsIFdpbGxpYW0gSi4=|https://frl.publisso.de/adhoc/creator/VGVwZWwsIE1hcnRpbg==
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Else Kröner-Fresenius Stiftung |
  3. Sonnenfeld-Stiftung |
  4. Ingeniør K.A. Rohde og Hustrus Legat |
  5. Tømrermester Alfred Andersen og Hustrus Fond |
  6. European Union |
  7. Danish Council for Independent Research (Det Frie Forskningsråd) |
1000 Fördernummer
  1. -
  2. -
  3. -
  4. -
  5. -
  6. FP7-HEALTH-2009-2.4.5-2 SYSKID; INTERREG 4A, number 62-1.2-10
  7. 10-084667
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
  5. -
  6. -
  7. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Else Kröner-Fresenius Stiftung |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Sonnenfeld-Stiftung |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Ingeniør K.A. Rohde og Hustrus Legat |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Tømrermester Alfred Andersen og Hustrus Fond |
    1000 Förderprogramm -
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm -
    1000 Fördernummer FP7-HEALTH-2009-2.4.5-2 SYSKID; INTERREG 4A, number 62-1.2-10
  7. 1000 joinedFunding-child
    1000 Förderer Danish Council for Independent Research (Det Frie Forskningsråd) |
    1000 Förderprogramm -
    1000 Fördernummer 10-084667
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404825.rdf
1000 Erstellt am 2017-10-05T11:37:02.734+0200
1000 Erstellt von 122
1000 beschreibt frl:6404825
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Jun 27 10:55:07 CEST 2018
1000 Objekt bearb. Wed Jun 27 10:55:07 CEST 2018
1000 Vgl. frl:6404825
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404825 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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