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WeightNameValue
1000 Titel
  • MicroRNAs Regulate Cellular ATP Levels by Targeting Mitochondrial Energy Metabolism Genes during C2C12 Myoblast Differentiation
1000 Autor/in
  1. Siengdee, Puntita |
  2. Trakooljul, Nares |
  3. Murani, Eduard |
  4. Schwerin, Manfred |
  5. Ponsuksili, Siriluck |
  6. Wimmers, Klaus |
1000 Erscheinungsjahr 2015
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-05-26
1000 Erschienen in
1000 Quellenangabe
  • 10(5):e0127850
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2015
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.1371/journal.pone.0127850 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444189/ |
1000 Ergänzendes Material
  • http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127850#sec025 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • In our previous study, we identified an miRNA regulatory network involved in energy metabolism in porcine muscle. To better understand the involvement of miRNAs in cellular ATP production and energy metabolism, here we used C2C12 myoblasts, in which ATP levels increase during differentiation, to identify miRNAs modulating these processes. ATP level, miRNA and mRNA microarray expression profiles during C2C12 differentiation into myotubes were assessed. The results suggest 14 miRNAs (miR-423-3p, miR-17, miR-130b, miR-301a/b, miR-345, miR-15a, miR-16a, miR-128, miR-615, miR-1968, miR-1a/b, and miR-194) as cellular ATP regulators targeting genes involved in mitochondrial energy metabolism (Cox4i2, Cox6a2, Ndufb7, Ndufs4, Ndufs5, and Ndufv1) during C2C12 differentiation. Among these, miR-423-3p showed a high inverse correlation with increasing ATP levels. Besides having implications in promoting cell growth and cell cycle progression, its function in cellular ATP regulation is yet unknown. Therefore, miR-423-3p was selected and validated for the function together with its potential target, Cox6a2. Overexpression of miR-423-3p in C2C12 myogenic differentiation lead to decreased cellular ATP level and decreased expression of Cox6a2 compared to the negative control. These results suggest miR-423-3p as a novel regulator of ATP/energy metabolism by targeting Cox6a2.
1000 Sacherschließung
lokal Energy metabolism
lokal Mitochondria
lokal Transfection
lokal MicroRNAs
lokal Gene expression
lokal Muscle differentiation
lokal Cell differentation
lokal Myoblasts
1000 Fachgruppe
  1. Agrarwissenschaften |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/U2llbmdkZWUsIFB1bnRpdGE=|https://frl.publisso.de/adhoc/creator/VHJha29vbGp1bCwgTmFyZXM=|https://frl.publisso.de/adhoc/creator/TXVyYW5pLCBFZHVhcmQ=|https://frl.publisso.de/adhoc/creator/U2Nod2VyaW4sIE1hbmZyZWQ=|https://frl.publisso.de/adhoc/creator/UG9uc3Vrc2lsaSwgU2lyaWx1Y2s=|http://orcid.org/0000-0002-9523-6790
1000 Förderer
  1. German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) |
  2. FBN (Research Institute for the Biology of Farm Animals) |
1000 Fördernummer
  1. PO753/7-1; WI 1754/10-14
  2. -
1000 Förderprogramm
  1. -
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) |
    1000 Förderprogramm -
    1000 Fördernummer PO753/7-1; WI 1754/10-14
  2. 1000 joinedFunding-child
    1000 Förderer FBN (Research Institute for the Biology of Farm Animals) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6404927.rdf
1000 Erstellt am 2017-10-11T11:19:56.471+0200
1000 Erstellt von 122
1000 beschreibt frl:6404927
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 28 11:31:36 CEST 2018
1000 Objekt bearb. Mon May 28 11:31:36 CEST 2018
1000 Vgl. frl:6404927
1000 Oai Id
  1. oai:frl.publisso.de:frl:6404927 |
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