12917_2015_Article_489.pdf 1,12MB
1000 Titel
  • Extracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells
1000 Autor/in
  1. Bauer, Isabel |
  2. Günther, Juliane |
  3. Wheeler, Thomas T. |
  4. Engelmann, Susanne |
  5. Seyfert, Hans-Martin |
1000 Erscheinungsjahr 2015
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-07-30
1000 Erschienen in
1000 Quellenangabe
  • 11: 172
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2015
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Considerably divergent data have been published from attempts to model the E. coli vs. S. aureus specific immune reaction of the udder using primary cultures of bovine mammary epithelial cells from cows (pbMEC). Some groups reported a swift, strong and transient inflammatory response against challenges with E. coli and only a weak and retarded response against S. aureus, in agreement with the respective reaction of the udder. Others found almost the reverse. Presence or absence of fetal calf serum distinguished the experimental setting between both groups. We examined here if this causes the divergent reaction of the pbMEC towards both pathogen species. We challenged pbMEC with proteins from heat killed E. coli or S. aureus pathogens or purified TLR2 and TLR4 ligands. The stimuli were applied in normal growth medium with (SM10) or without (SM0) 10 % fetal calf serum, or in the basal medium supplemented with 10 mg/ml milk proteins (SM Milk). RESULTS: Withdrawal of FCS slowed down and decreased the extent by which E. coli or LPS enhanced the expression of cyto- and chemokine encoding genes through impaired TLR4 signalling but enforced their expression during stimulation with S. aureus. SM Milk strongly quenched the induction of those genes. S. aureus strain specific differences in the reaction of the pbMEC could only be recorded in SM0. NF-κB factors were activated by E. coli in all stimulation media, but only to a small extent by S. aureus, solely in SM0. Purified ligands for TLR2 stimulated expression of those genes and activated NF-κB equally well in SM10 and SM0. The mRNA destabilizing factor tristetraproline was only induced by E. coli in SM10 and by purified PAMPs. CONCLUSIONS: Our data cross validate the correctness of previously published divergent data on the pathogen-specific induction of key immune genes in pbMEC. The differences are due to the presence of FCS, modulating signalling through TLR4 and TLR-unrelated pathogen receptors. S. aureus does not substantially activate any TLR signalling in MEC. Rather, receptors distinct from TLRs perceive the presence of S. aureus and control the immune response against this pathogen in MEC.
1000 Sacherschließung
lokal NF-κB
lokal Mastitis
lokal Cattle
lokal Escherichia coli
lokal Serum
lokal Inflammation
lokal Innate immune response
lokal TLR signalling
lokal Staphylococcus aureus
1000 Fachgruppe
  1. Biologie |
  2. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
1000 Fördernummer
  1. Se 14/3-1; TRR34
1000 Förderprogramm
  1. Researcher Group FOR585
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm Researcher Group FOR585
    1000 Fördernummer Se 14/3-1; TRR34
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405047.rdf
1000 Erstellt am 2017-10-16T18:03:08.554+0200
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1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 20:46:43 CET 2020
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