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1000 Titel
  • Comparing Two Intestinal Porcine Epithelial Cell Lines (IPECs): Morphological Differentiation, Function and Metabolism
1000 Autor/in
  1. Nossol, Constanze |
  2. Barta-Böszörményi, Anicò |
  3. Kahlert, Stefan |
  4. Zuschratter, Werner |
  5. Faber-Zuschratter, Heidi |
  6. Reinhardt, Nicole |
  7. Ponsuksili, Siriluk |
  8. Wimmers, Klaus |
  9. Diesing, Anne-Kathrin |
  10. Rothkötter, Hermann-Josef |
  11. Wimmers, Klaus |
1000 Erscheinungsjahr 2015
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-07-06
1000 Erschienen in
1000 Quellenangabe
  • 10(7): e0132323
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2015
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0132323 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493080/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The pig shows genetical and physiological resemblance to human, which predestines it as an experimental animal model especially for mucosal physiology. Therefore, the intestinal epithelial cell lines 1 and J2 (IPEC-1, IPEC-J2) - spontaneously immortalised cell lines from the porcine intestine - are important tools for studying intestinal function. A microarray (GeneChip Porcine Genome Array) was performed to compare the genome wide gene expression of IPECs. Different significantly up-regulated pathways were identified, like “lysosome”, “pathways in cancer”, “regulation of actin cytoskeleton” and “oxidative phosphorylation” in IPEC-J2 in comparison to IPEC-1. On the other hand, “spliceosome”, “ribosome”, “RNA-degradation” and “tight junction” are significantly down-regulated pathways in IPEC-J2 in comparison to IPEC-1. Examined pathways were followed up by functional analyses. ATP-, oxygen, glucose and lactate-measurement provide evidence for up-regulation of oxidative phosphorylation in IPEC-J2. These cells seem to be more active in their metabolism than IPEC-1 cells due to a significant higher ATP-content as well as a higher O2- and glucose-consumption. The down-regulated pathway “ribosome” was followed up by measurement of RNA- and protein content. In summary, IPEC-J2 is a morphologically and functionally more differentiated cell line in comparison to IPEC-1. In addition, IPEC-J2 cells are a preferential tool for in vitro studies with the focus on metabolism.
1000 Sacherschließung
lokal Glucose metabolism
lokal Caco-2 cells
lokal Tight junctions
lokal Epithelial cells
lokal Microarrays
lokal Cell metabolism
lokal Gastrointestinal tract
lokal Microvilli
1000 Fachgruppe
  1. Biologie |
  2. Agrarwissenschaften |
  3. Gesundheitswesen |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/Tm9zc29sLCBDb25zdGFuemU=|https://frl.publisso.de/adhoc/creator/QmFydGEtQsO2c3rDtnJtw6lueWksIEFuaWPDsg==|https://frl.publisso.de/adhoc/creator/S2FobGVydCwgU3RlZmFu|https://frl.publisso.de/adhoc/creator/WnVzY2hyYXR0ZXIsIFdlcm5lcg==|https://frl.publisso.de/adhoc/creator/RmFiZXItWnVzY2hyYXR0ZXIsIEhlaWRp|https://frl.publisso.de/adhoc/creator/UmVpbmhhcmR0LCBOaWNvbGU=|https://frl.publisso.de/adhoc/creator/UG9uc3Vrc2lsaSwgU2lyaWx1aw==|https://frl.publisso.de/adhoc/creator/V2ltbWVycywgS2xhdXM=|https://frl.publisso.de/adhoc/creator/RGllc2luZywgQW5uZS1LYXRocmlu|https://frl.publisso.de/adhoc/creator/Um90aGvDtnR0ZXIsIEhlcm1hbm4tSm9zZWY=|http://orcid.org/0000-0002-9523-6790
1000 Förderer
  1. German Research Council (DFG) |
1000 Fördernummer
  1. Ro 743/3-3
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Council (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer Ro 743/3-3
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405121.rdf
1000 Erstellt am 2017-10-20T17:50:13.099+0200
1000 Erstellt von 218
1000 beschreibt frl:6405121
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 07 12:07:33 CEST 2018
1000 Objekt bearb. Mon May 07 12:07:32 CEST 2018
1000 Vgl. frl:6405121
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405121 |
1000 Sichtbarkeit Metadaten public
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