WeightNameValue
1000 Titel
  • Overlapping functions of stonin 2 and SV2 in sorting of the calcium sensor synaptotagmin 1 to synaptic vesicles
1000 Autor/in
  1. Kaempf, Natalie |
  2. Kochlamazashvili, Gaga |
  3. Bajjalieh, Sandra M. |
  4. Kononenko, Natalia L. |
  5. Puchkov, Dmytro |
  6. Maritzen, Tanja |
  7. Haucke, Volker |
1000 Erscheinungsjahr 2015
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-06-09
1000 Erschienen in
1000 Quellenangabe
  • 112(23): 7297-7302
1000 FRL-Sammlung
1000 Verlagsversion
  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466747/ |
  • http://dx.doi.org/10.1073/pnas.1501627112 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Neurotransmission involves the calcium-regulated exocytic fusion of synaptic vesicles (SVs) and the subsequent retrieval of SV membranes followed by reformation of properly sized and shaped SVs. An unresolved question is whether each SV protein is sorted by its own dedicated adaptor or whether sorting is facilitated by association between different SV proteins. We demonstrate that endocytic sorting of the calcium sensor synaptotagmin 1 (Syt1) is mediated by the overlapping activities of the Syt1-associated SV glycoprotein SV2A/B and the endocytic Syt1-adaptor stonin 2 (Stn2). Deletion or knockdown of either SV2A/B or Stn2 results in partial Syt1 loss and missorting of Syt1 to the neuronal surface, whereas deletion of both SV2A/B and Stn2 dramatically exacerbates this phenotype. Selective missorting and degradation of Syt1 in the absence of SV2A/B and Stn2 impairs the efficacy of neurotransmission at hippocampal synapses. These results indicate that endocytic sorting of Syt1 to SVs is mediated by the overlapping activities of SV2A/B and Stn2 and favor a model according to which SV protein sorting is guarded by both cargo-specific mechanisms as well as association between SV proteins.
1000 Sacherschließung
lokal neurotransmission
lokal endocytosis
lokal knockout mice
lokal synaptic vesicle protein sorting
lokal calcium sensor
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S2FlbXBmLCBOYXRhbGll|https://frl.publisso.de/adhoc/creator/S29jaGxhbWF6YXNodmlsaSwgR2FnYQ==|https://frl.publisso.de/adhoc/creator/QmFqamFsaWVoLCBTYW5kcmEgTS4=|https://frl.publisso.de/adhoc/creator/S29ub25lbmtvLCBOYXRhbGlhIEwu|http://orcid.org/0000-0001-8341-4847|http://orcid.org/0000-0003-4993-0340|http://orcid.org/0000-0003-3119-6993
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
1000 Fördernummer
  1. SFB958/A01
1000 Förderprogramm
  1. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SFB958/A01
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405485.rdf
1000 Erstellt am 2017-11-24T10:32:10.846+0100
1000 Erstellt von 122
1000 beschreibt frl:6405485
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet 2022-08-18T07:48:59.920+0200
1000 Objekt bearb. Mon Dec 07 12:54:58 CET 2020
1000 Vgl. frl:6405485
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405485 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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