WeightNameValue
1000 Titel
  • High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy
1000 Autor/in
  1. Demers, Jean-Philippe |
  2. Habenstein, Birgit |
  3. Loquet, Antoine |
  4. Vasa, Suresh Kumar |
  5. Giller, Karin |
  6. Becker, Stefan |
  7. Baker, David |
  8. Sgourakis, Nikolaos G. |
  9. Lange, Adam |
1000 Erscheinungsjahr 2014
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2014-09-29
1000 Erschienen in
1000 Quellenangabe
  • 5: 4976
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251803/ |
  • http://doi.org/10.1038/ncomms5976 |
1000 Ergänzendes Material
  • https://www.nature.com/articles/ncomms5976#supplementary-information |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • We introduce a general hybrid approach for determining the structures of supramolecular assemblies. Cryo-electron microscopy (cryo-EM) data define the overall envelope of the assembly and rigid-body orientation of the subunits while solid-state nuclear magnetic resonance (ssNMR) chemical shifts and distance constraints define the local secondary structure, protein fold and inter-subunit interactions. Finally, Rosetta structure calculations provide a general framework to integrate the different sources of structural information. Combining a 7.7-Å cryo-EM density map and 996 ssNMR distance constraints, the structure of the type-III secretion system needle of Shigella flexneri is determined to a precision of 0.4 Å. The calculated structures are cross-validated using an independent data set of 691 ssNMR constraints and scanning transmission electron microscopy measurements. The hybrid model resolves the conformation of the non-conserved N terminus, which occupies a protrusion in the cryo-EM density, and reveals conserved pore residues forming a continuous pattern of electrostatic interactions, thereby suggesting a mechanism for effector protein translocation.
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/RGVtZXJzLCBKZWFuLVBoaWxpcHBl|https://frl.publisso.de/adhoc/creator/SGFiZW5zdGVpbiwgQmlyZ2l0|https://frl.publisso.de/adhoc/creator/TG9xdWV0LCBBbnRvaW5l|https://frl.publisso.de/adhoc/creator/VmFzYSwgU3VyZXNoIEt1bWFy|https://frl.publisso.de/adhoc/creator/R2lsbGVyLCBLYXJpbg==|https://frl.publisso.de/adhoc/creator/QmVja2VyLCBTdGVmYW4=|https://frl.publisso.de/adhoc/creator/QmFrZXIsIERhdmlk|https://frl.publisso.de/adhoc/creator/U2dvdXJha2lzLCBOaWtvbGFvcyBHLg==|http://orcid.org/0000-0002-7534-5973
1000 Label
1000 Förderer
  1. Max Planck Society |
  2. Leibniz-Institut für Molekulare Pharmakologie |
  3. Deutsche Forschungsgemeinschaft |
  4. NSERC of Canada |
  5. Fondation Bettencourt Schueller |
  6. EMBO |
  7. National Institutes of Health |
  8. European Union |
1000 Fördernummer
  1. -
  2. -
  3. -
  4. -
  5. -
  6. -
  7. 1 R01 GM092802-01
  8. 261863
1000 Förderprogramm
  1. -
  2. -
  3. Emmy Noether Fellowship
  4. -
  5. -
  6. -
  7. Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases
  8. Seventh Framework Program under Grant Agreement (Bio-NMR)
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Max Planck Society |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Leibniz-Institut für Molekulare Pharmakologie |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm Emmy Noether Fellowship
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer NSERC of Canada |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Fondation Bettencourt Schueller |
    1000 Förderprogramm -
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer EMBO |
    1000 Förderprogramm -
    1000 Fördernummer -
  7. 1000 joinedFunding-child
    1000 Förderer National Institutes of Health |
    1000 Förderprogramm Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases
    1000 Fördernummer 1 R01 GM092802-01
  8. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm Seventh Framework Program under Grant Agreement (Bio-NMR)
    1000 Fördernummer 261863
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405534.rdf
1000 Erstellt am 2017-11-28T12:22:49.691+0100
1000 Erstellt von 25
1000 beschreibt frl:6405534
1000 Bearbeitet von 288
1000 Zuletzt bearbeitet 2022-08-18T07:48:32.241+0200
1000 Objekt bearb. Thu Mar 04 08:09:06 CET 2021
1000 Vgl. frl:6405534
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405534 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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